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PATH3309 > Ancillary Studies > Flashcards

Ancillary Studies Flashcards

1
Q

What 3 people do you need in path lab?

A

Scientist
Pathologist
Laboratory

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2
Q

What are all techniques?

A 
Special stains
Immunohistochemistry
Molecular testing
Flow cytometry
Cytogenetics
Fresh tissue
Electron
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3
Q

What are special stains?

A

Visualise details ofthe cell

Cell of origin by morphology and characteristics

H&E

Mucin stains

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4
Q

What is mucin produced by?

A

Glands or adenocarcinoma

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5
Q

What are some mucin stains?

A

Mucicarmine

Alcian blue

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6
Q

You see mucin what you got?

A

Adenocarcinoma

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7
Q

What happens when cytokeratin stains positive?

A

Carcinoma

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8
Q

What is a cell block?

A

FNA
Concentrate cells
Process cells like tissue biopsy

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9
Q

What is cytokeratin IHC?

A

CK20 stains cytokeratin

Means epithelial malignancy

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10
Q

What are some neuroendocrine markers?

A

Synapthophysin
Chromogranin
MNF-116
CD56

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11
Q

What does CDX2 stain?

A

Gut + or neoplastic pancreas cells

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12
Q

What does TTF-1 stain?

A

Lung and thyroid cancer

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13
Q

In what tissue is immunohistochemistry carried out?

A

Formalin fixed paraffin embedded tissue

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14
Q

What is peroxidase-antiperoxidase-biotin-avidin conjugate color?

A

Brown

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15
Q

What does trastuzumabb do?

A

Herceptin
Monoclonal antibody
Targets HER2 binding
Prevents promotion of proliferation by upregulating p27

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16
Q

What type of cell can you conduct in Situ hybridization on?

A

Non-dividing cells
Air-dried or formalin - fixed specimens
Facilitate detection of numeric abnormalities

17
Q

What components are involved in molecular pathology?

A

DNA
RNA
Protein

18
Q

What are some molecular techniques?

A

PCR
DNA sequencing
other

PATH3309 (14 decks)

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