Analysis of Gene Expression

Question 1

scRNA-seq (single cell) difficulties

Answer 1

RNA has short half life
Transcriptional bursting
No poly-A tails
Gram +/-

Question 2

What is useful about scRNA-seq?

Answer 2

Can detect unique profiles of log phase growth
Subpopulations easier to study
Detect spatial organization of transcription and or RNAs
Can analyze when cells response to regulatory cascade and the order of the cascade

Question 3

What does flhCD do?

Answer 3

Regulate expression of fliaA (sigma F) for flagellar movement