Analysis and follow-up Flashcards
CML: How many cultures, what stimulation, how long cultured?
2
None
1 day and 2 days, can do same day smear if urgent.
CML: How many cells analysed and scored by G-banding if
a) normal
b) abnormal
a) 10 + 10
b) 3 + 7
CML: How many FISH Mets and Interphase cells need to be scored to report?
Mets - 5
Interphases: 100
CML: If there is no evidence of Ph+ in 20 cells screened yet a clinical diagnosis of CML is still suspected, what should you do?
FISH for BCR-ABL fusion; translocation may be cryptic
CML, follow-up testing: How frequently should samples be analysed? When should you switch from BM to PB?
3mo, 6mo and every 6mo after until CCgR achieved and confirmed in a second sample. PH+ then screened every 6 months by RQ-PCR
Switch to PB once CCgR achieved.
CML: How many cells analysed and scored AT FOLLOW-UP by G-banding if
a) normal (Ph-)
b) abnormal (Ph+)
b) evidence of relapse
a) 20
b) 30
c) 5 + 5
CML: How many FISH Mets and Interphase cells need to be scored AT FOLLOW-UP to report if
a) normal (Ph-)
b) Abnormal (Ph+)
c) insufficient metaphases
a) 30 nuclei
b) 20 nuclei
c) 100 nuclei
CML: What should be stated on the report about the type of cells screened? Why?
The report should state whether selected or unselected nuclei have been screened.
There are varying proportions of normal lymphocytes; screening neutrophils/granulocytes is more representative of the true count.
CML: what additional clonal abnormalities can be detected in Ph+ cells?
+8
abn 7
Sex chromo abn
MPN: what excludes a diagnosis of MPN?
Ph+
MPN: What molecular analysis is performed?
PDGFRA PDGFRB FGFR1 JAK2 - V617F CALR MPL
AML: What are common findings in therapy-related AML?
7q-
5q-
MLL translocations
21q22 translocations (RUNX1)
AML: What is the preferred sample for testing?
BM
AML: How many cultures, what stimulation, how long cultured?
2
none, or IL3/GMCSF can help
1day and 2 days
AML: How many cells analysed and scored by G-banding if
a) normal
b) abnormal
a) 10 + 10
b) 5 + 5
How is a ‘clone’ defined by ISCN?
2 or more cells with gains/structural abnormalities
3 or more cells with losses
AML: What other findings is inv(16) associated with?
del9q
+22
AML: What should be suspected if the in infant AML?
MLL translocations
AML: What is the MECOM probe used to screen for?
3q duplications
Which references are used for classification of CML, adult AML and childhood AML, respectively?
Baccarani et al, 2009
Grimwade et al, 1998 or 2010
Harrison et al, 2010
What sample is required for MDS analysis?
BM - PB is no good
ALL:How is high hyperdiploidy defined?
51-65 chromosomes
ALL: How is low hypodiploidy defined?
30-39 chromosomes
ALL: How is Near haploidy defined?
23-29 chromosomes
