anaesthetic gas and blood measurements Flashcards

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1
Q

draw a capnography trace and explain the phases…

A

Capnography displays a trace of exhaled CO2.
phase 1 = initially deadspace is expired which contains no CO2
phase 2 = CO2 expired from alveolar gas and mixed with dead space gas, sharp rise as more alveolar gases empty and mix
phase 3 = pure alveolar gas - platau with slight increase. The peak gives a measurement of ETCO2
phase 0 or 4 = inspiration, no CO2 so sudden fall.

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2
Q

what might cause and increase/ decrease in ETCO2

A
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3
Q

what does this trace show..

A

Patient making own resp efforts over the top of the ventilator

either switch to spontaneous mode or give more muscle relaxant or may tolerate pressure support better.

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4
Q

draw a capnography to show airway obstruction..

A
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5
Q

what is the importance of CO2 monitoring?

A

Allows useful info about airway and breathing.

airway - placement of tube e.g. in intubation, throughout operation.

breathing - gives info on level of arterial CO2 e.g. if high or low can help diagnosis. Adequacy of ventilation. pattern may indicate bronchospasm

circulation - also info on circulation e.g. increase in CO2 is positive in cardiac arrest. sudden drop may be P.E

detection of rebreathing and needing to change soda lime/ increase flow.

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6
Q

if you could have one piece of monitoring equiptment what would it be?

A

capnography - gives a lot of useful info and not invasive, simple to use and set up.

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7
Q

what are the various methods for measuring CO2 in a gas mixture?

A

most common = infrared spectrometry
others..
* Raman spectrometry
* mass spectrometry
* colourimetry - use of litmus paper
* gas chromatography
* savinghaus electrode

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8
Q

how does an infrared analyser work?

A

Based on fact that all molecules with 2 dissimilar atoms will absorb infrared light at a particular wavelength, for CO2 this is 4.3um - knowing this wavelength we can expose the molecules to this and measure how much light was absorbed using beers law.
Beers law = amount absorbed is proportional to concentration of gas molecules in the mixture

Uses a light source which emits infrared light from hot wire, filter to produce a specific wavelength of light, exposes this to a gas chamber of known constant size (lamberts is constant) and then has a photodetector to measure amount of light leaving the chamber. ( a lens focuses light onto this photodetector)

Reference chamber
during the process the light also passes through a reference chamber of known concentration. The absorptions are compared by the microprocessor this helps to remove any errors such as non-continous outputs by light source, ambient light influence, changes to functioning of the detector.

microprocessor displays info on a screen.

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9
Q

what is beers and lamberts law? including equation

A

Beers law = amount absorbed is proportional to concentration of gas molecules in the mixture

Lambert law = amount absorbed is proportional to the length the light has to travel - this is kept constant

amount absorbed = ebc
c = conc, b = length passed,
e = molar absorbance - constant per substance per mole.

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10
Q

what is the role of the interference filter..

A

A filter is placed between hot wire infrared source and reference chamber
this ensures only a specific wavelength of light is allowed to pass that is absorbed by the gas of interest.
therefore the amount absorbed can be said to be directly proportional to the conc of that gas.

otherwise, light of other wavelengths will be absorbed by other gases and this wont be an accurate measure

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11
Q

what are the sample chamber walls made from?

A

crystals of silver bromide or salphire
doesnt absorb light of this wavelength e.g. glass would absorb infrared light.

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12
Q

how does the photodetector work?

A

using a thermopile
as light shines on the thermopile, it is heated. The seabek effect is used where 2 dissimilar metals placed next to eachother which when exposed to a temperature will produce a voltage. this voltage is proportional to the temp change.
hence depending on the output voltage, the amount of light detected and hence absorbed can be deduced.

e.g. less CO2 in sample, the more light passing through, more temp at thermopile, higher voltage.

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13
Q

what is the difference between capnography and capnometry?

A

capnography = display of the ETCO2 waveform
capnometry is the measure of CO2 conc

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14
Q

what are the 2 methods gases can be sampled from the breathing system?

A

Side stream or Main stream analysers.

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15
Q

Tell me about side stream gas analysis…

A

Consists of a small tube of 1.2mm internal diameter, which removes a small samples from the expiratory limb of the breathing circuit.

This is taken at a flow of 150ml/min to be anlysed

it is made from teflon which is impermeable to CO2 and doesnt react with anaesthetic gases.

The closer this is placed to the trachea, the more accurate.

The gas is later added back to the circuit

PROS:
* less monitoring equiptment needed near patient end so more practical
* allows room for larger analyser that can measure other agents too.
* less bulky so can be used in prone.
* no deadspace

CONS:
* water vapour can interfer with CO2 and condense in the circuit. water trap required
* pressure drop may effect measurements
* delay in measurement readings - 2 to 3 secs

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16
Q

tell me about the mainstream gas analyser for CO2

A

A gas analyser with sapphire windows is attached directly to breathing circuit
PROS:
therefore more bulky and limits positioning e.g. prone.
Also limited to size and so usually only for capnography.
CONS:
however there is less of a time delay and can be good for transport.
Adds deadspace
It is also affected by water vapour making it unreliable, by heating the element is can reduce this problem however this poses a risk to the patient.

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17
Q

what determines the delay in gas conc analysis

A

side stream vs main - side stream has more delay
transit time - length of tube and flow rate
diameter of the tube - the smaller, the less delay

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18
Q

what are the problems with infrared spectroscopy?

A

water vapour
* water has a wide infrared absorption so can absorb certain wavelengths and cause inaccuracies
* use of a water trap needed and hygroscopic tubing
* also can condense and physically block tube - position tube upwards to prevent droplets getting in

N20
* collision broadening
* CO2 absorbs infrared light, this molecule can collide with N20 and energy is transferred to N20 and CO2 can absorb more light - hence appears as if there is more CO2 than there is.
* modern machines compensate for this by knowing how much N20 is used.

O2
* cant be measured by infrared spec

atmospheric pressure
* changes to ambient pressure will change the partial pressure of CO2

lag time - to get to analyser and be analysed.

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19
Q

what are the advantages of IR spectrometry?

A

Can simulataneously measure different gases at same time - use a filter that rotates

relatively inexpensive e.g. compared to mass spec

continuous measurements

gases can be added back into the circuit - i.e. doesnt change composion

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20
Q

what is Raman Spectrometry?

A

Gas from breathing system is exposed to a monochromatic light from an argon laser

the energy absorbed from the intermolecular bonds is re-emitted at a new wavelength

the wavelength shift and scattering can be used to measure conc because the wavelength is specific to each gas.

can also be used to measure O2 as well as N20 and voltatiles

however expensive and complex

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21
Q

how does Mas spectrometry work?

A

sample of gas taken and bombarded with electrons to cause it to fragment and ionise. (using anode and cathode)

These molecules are then accelarated by a magnetic field in a vacuum chamber

the distance travelled is proportional to their charge:mass ratio i.e. smaller charged ones will travel further.

By comparing amounts of different fragments, the amount of the parent compound can be determined.

Each gas usually has its own finger print

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22
Q

what are the pros and cons of mass spec?

A

very reliable, accurate and can measure many gases including O2

however bulky, expensive, time consuming, changes composition of gas

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23
Q

give an overview of how each anaesthetic gas is measured?

A

usually infrared absorption spectrometry including CO2, volatile, N20

however O2 by paramagnetic O2 analysis usually and sometimes by galavanic fuel cell

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24
Q

how does infrared spectrometry measure anaesthetic gases e.g. sevo

A

same method as CO2 - i.e. infrared spectrometry however each gas has its own specific wavelength
CO2 - 4.3um
N20 - 4.6um
H20 - multiple wavelengths from 1.4 to 6.3
anaesthetic gases 8-9um

either measured via separate chambers and filters
or by the same chamber exposed to a rotating filter of different wavelengths

each anaesthetic gas has its own absorbance spectral shape e.g. absorbance of y and wavelength on x axis. this can be compared to a memory stored in the machine to detect the agent present and amount of absorbance is proportional to amount of agent

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25
Q

how is CO2 measured in anaesthetic practice?

A

Gas analysis - infrared spectrometry and capnography

blood gas analysis - servinghaus electrode

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26
Q
A

A = normal
B = sloped phase 3 , no plataeu -airway obstruction e.g. COPD, bronchospasm
C = resp efforts over the top
D = raised baseline, exhausted soda lime or rebreathing
E = increasing ETCO2 - hypoventilation, MH, laparoscopic surgery

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27
Q

what does this represent…

A

cardiac oscilations - due to heart beat

28
Q

what are the uses of capnography in cardiac arrest?

A

placement of ET tube

monitoring ventilation rate

monitoring quality of chest compression - should be more than 2.5kpa

monitoring ROSC

29
Q

what are the uses of capnography in non-theatre areas…

A

ET in correct place vs oesophageal intubation in ITU
adequacy of ventilation and cardiac output in ITU.

also
during percutaneous tracheostomy insertion
can be used as a surrogate marker for arterial CO2 for those with head injuries
monitoring for sedation either in ED or scan

30
Q

outline different methods of O2 gas conc measurement…

A

Can be classified into
electrical
* Fuel cell
* clark electrode

non electrical
* paramagnetic O2 analyser - main one used on anaesthetic machine insp and exp limb
* mas spec - used in research

31
Q

how does the fuel cell work..

A

2 electrodes with shared solution completing a circuit. Ammeter to measure current.
(LAG = lead anode, gold cathode)

  1. anode - lead. reacts with OH in solution to produce lead oxide and electrons. e- flow around to cathode
  2. cathode - reduces oxygen to produce OH- ions.
  3. OH- ions carry current to anode to complete circuit

reactions…
anode: Pb + 2OH- –> PbO + H20 + 2e-
cathode: O2 + 2H20 + 4e- –> 4OH-

the more O2 , the more reduced at the cathode, the quicker electrons can flow hence current is proportional to O2 conc

32
Q

how is the fuel cell calibrated?

A

against 2 known samples of 21% and 100% O2
daily

this is because current output varies with life of cell.

33
Q

how quick is a fuel cell?

A

takes around 20-30seconds

34
Q

what limits the lifespan of a fuel?

A

the lead anode is oxidised with time/ exposure to O2

if left exposed to high O2 it will deplete in a few weeks

35
Q

pros and cons of the fuel cell..

A

pros:
* cheap, compact, no power source needed

cons:
* gas mixtures containing N20 can damage it as it reacts with lead anode and produces N2 gas which exposes components to pressure
* redox reaction at cathode is temp sensitive so need to keep temp constant
* slow response time.
* needs regular calibration, maintainance and replacement of lead anode.

36
Q

how does a paramagnetic O2 analyser work?

A

O2 is paramagnetic i.e. attracted to strong magnetic field. Due to its unpaired e- in outer shell.

The O2 is exposed to a strong magnetic field and therefore is attracted to the other side and gas flows. this will cause 2 suspended dumbells, connected to a bar and filled of N2 gas to rotate. the degree of rotation is proportional to amount of O2 present.

there are a number of ways to measure this rotation
1. mirror and light beam - the angle of reflection of the light beam will tell you the degree of rotation of dumb bells.
2. null deflection - a rotating motor preventing rotation, the current needed to resist rotation is proportional to amount of O2

37
Q

what is a pulsed field paramagnetic analyser…

A

newer version of paramagnetic - no longer use dumbells but a pressure transducer

reference chamber and compare pressure changes…
this works via having 2 chambers - one unknown and one reference of known O2.
pulsed magnetic field is applied - when O2 molecules move, the pressure drops, displacing the diaphragm.
A pressure transducer can compare the pressure drop in each chamber to determine O2 conc - the pressure drop is proportional to speed of O2 flow.

accurate and continuous, less prone to drift and more robust than classic paramagentic analysers
however can still be affected by water vapour

38
Q

pros and cons of paramagnetic O2 analyser..

A

Pros
* quick
* no need for frequent calibration/ replacement
* continous

cons:
* water vapour can cause inaccuracies through stickign and altering ease of movement of the balls.
* needs calibration
* needs power supply
* magnetic properties of other gases could cause interference however not significant as O2 is much more paramagnetic

39
Q

what is the main differences between clark and fuel cell electrode..

A

clark - needs voltage
different solutions and voltage

40
Q

how does gas chromatography work?

A

separation of gases by passing through a column with a mobile and stationary phase
different gases travel at different speeds depending on solubility in each phase
hence separated and detected by using known reference

41
Q

why are inspiratory and expiratory volume measurements not the same?

A

presence of water vapour from lungs

42
Q

give an overview of what blood gas analysers can measure..

A

direct measurements
* O2 - fuel / clark
* pH -pH electrode
* CO2 - serveringhaus
* other electrolytes - Na, K, glucose

derived = HCO3, BE, O2 sats

43
Q

what is the difference between an amperiometric and potentiometric technique

A

measures a current vs voltage
e.g. O2 measures current
pH and CO2 - measure voltage

44
Q

what are the indications of blood gas monitoring?

A

respiratory or metabolic acid/ base derrangements

see response to treatment in respiratory disease e.g. before/after NIV

quick way to check K+ and Hb before official lab results are back

45
Q

how is an ABG sample obtained and analysed?

A

usually from radial arterial, also femoral
asceptic tecnique - wash hands, gloves, chlorprep
22 G needle or via arterial line
heparin filled syringe - remove heparin first
can use a capillary tube or syringe

after sample taken
* remove air bubbles
* reduce time to analysis
* deliver via inlet probe of gas analyser
* aspirates sample and heats to 37 degrees
* input patient details, FiO2 and temp

46
Q

what is a co-oximeter?

A

works in a similar way to pulse oximetry but on a blood sample

allows analysis of different forms of Hb e.g. oxy, deoxy, carboxy, methamoglobin
using a spectrometer of 4 different wave lengths

47
Q

how can the saturations of arterial blood be measured in sample of blood…

A
  1. co-oximetery measures directly
  2. using O2 sats dissociation curve - known PaO2 so can derive saturated Hb
48
Q

what is the difference between pulse oximeter and co-oximeter?

A

pulse oximeter - in vivo, non invasive, also measures pulse rate

co-oximeter also uses spectrophotometry but in vitro on blood sample. usually more types of hb

49
Q

explain how a polographic clark cell works..

A

(polargraphic clark = PC = platinum cathode )

2 electrodes - anode and cathode, connected in a circuit with common ionic solution ( KCl ) and wire containing ammeter.

  1. anode = Ag/AgCl
    Cl ions react with silver, to release e-. these flow around to cathode
  2. cathode = platinum
    e- reduce O2 to form OH- ions

reactions
Ag + Cl- –> AgCl + e-
O2 + H20 + 4e- –> 4OH-
overall
4Ag + 4KCl + O2 + 2H20 –> 4AgCl + 4KOH

the more O2 available, the quicker the reduction, more current can flow. hence the current measured is proportional to amount of O2. calibrated scales of known values used to determine ppO2

uses voltage of 0.6V
teflon membrane permeable to O2

50
Q

pros and cons of the clark electrode

A

pros
* compact
* accurate
* acceptable response time - but not quick enough for continous ins/exp gases
* slower depletion of electrodes than fuel cell - lasts longer 3yrs.

cons
- needs voltage
- needs frequent calibration
- membrane delicate, prone to damage and protein deposits which affects performance
- halothane can give high O2 readings, now membrane used impermeable to halothane.

51
Q
A
52
Q

explain how a pH electrode works?

A

2 half cells
reference electrode = silver/silver chloride + KCl / KOH solution. AgCl + e- –> Cl- + Ag (sometimes uses a calomel/ mercury electrode)

measuring electrode = silver / silver chloride electrode + 0.1M HCl buffer solution. pH sensitive glass in contact with blood. pH sensitive glass is negativley charged on both sides(silica) , H+ ions bind and need to be equal on both sides, causes HCL to dissolciate and Cl free to react with Ag. produces AgCl + e- .

kept at a constant temp
potential difference betwen 2 half cells is measured.

53
Q

what are the pros and cons of the pH electrode..

A

small, compact, accurate, resonable response time

cons
* affected by temp changes
* needs to be calibrated against 2 known solutions
* regular maintainance as membrane and electrode can deteriorate
* non continous

54
Q

tell me about the CO2 servinghaus electrode..

A

closely related to pH electrode
uses fact than CO2 and H+ have a tightly linked relationship

one added step - CO2 crosses CO2 permeable membrane (which is impermeable to H+) into a NaHCO3 solution
CO2 + H20 –> H+ + HCO3
this solution is in contact with pH sensitive glass and reference electrode.
H+ can be measured via pH electrodes.

voltage measured is proportional to H+ and hence CO2

55
Q

pros and cons of CO2 electrode..

A

small, compact, accurate

cons
slower response time - 2 to 3 mins - extra step in reacting CO2 and H20 and diffusion across membrane
membrane can become damaged/ covered in proteins
regular calibration

56
Q

which method of O2 measurement is used in modern gas analysers?

A

clark
(fuel cell used to be used on anaesthetic machine for gas measurements, now replaced by paramagnetic)

57
Q

tell me about transcutaneous O2 measurements..

A

continous non invasive measurement of O2 tension through skin. not used due to convience of pulse oximetry.

clark electrode applied to skin, heating element causes vasodilation and O2 can diffuse from blood out of skin to clark electrode for measurement

less accurate and can cause burns. but is a method of continuous non invasive measurement

58
Q

do you know any methods for continous blood gas measurements?

A

transcutaneous O2 measurements - clark on skin surface

intra-artial placement of a clark electrode - risks of thrombosis, infection and reduces accuracy of invasive BP monitoring

optical sensors coated in flurescent dyes - the intensity of the dye will veary with O2 and CO2 PP

jugular venous oximetry - pulse ox in IJV to measure brain sats.

59
Q

how are gas machines calibrated and maintained?

A

calibrations..
1 and 2 point calibrations at regular intervals automatically and whenever operator wants to perform.

maintainance - electrodes, membranes, solutions.

quality control - use known solutions to test accuracy

60
Q

what are teh sources of error for blood gas machines?

A

temperature
important to tell machine, the temp of patient as temp influences how well gases dissolve in solution and dissociation of acids. e.g. in hypothermic patient, more CO2 will be dissolved in solution, if heated up this will come out of solution and give higher pp so will appear hypercarbic when not. similarly acidotic

air bubbles
contain 21% O2 so will give falsely high readings

**heparin **
causes acidity of blood

delays in sample
respiration - production of CO2, use of O2 so changes

**machine problems **
* drift and inaccuracies - need recalibrating
* membrane damage - protein deposits

61
Q

how are errors in blood gas measurements reduced?

A

corrected for patients body temp
minimise delays
remove air bubbles

regular calibration and maintainance

62
Q

AABGI standards for gas monitoring…

A

during general anaesthesia
02 monitoring + alarms
Capnography - sedation and general
vapour analysis when used.

63
Q

what is a UV gas analyser..

A

used to measure halothane as this absorbs light in UV region
same principle as infrared spectrometry but UV light.

64
Q

what is meant by a double beam capnometer

A

the process used in infrared spectroscopy of capnography whereby a reference chamber is used.

allowing the device to compensate for changes in the light source or detector sensitivity, changes in light source output, improving accuracy.

65
Q

draw Capnograph for emphysema..

A

due to impaired alveolar ventilation and gas trapping/ closure of airways