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Liz's Microbiology Module 2 > Anaerobic GPRs > Flashcards

Anaerobic GPRs Flashcards

1
Q

Propionibacterium acnes

- Normal flora

A

Skin (contaminant)

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2
Q

Propionibacterium acnes

- GLC

A

GLC = aP

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3
Q

Propionibacterium acnes

- Diseases or infections it’s involved in

A

CNS shunt infections, endocarditis, prosthetic joint infections, eye
- Found in blood and CSF cultures after 5 days

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4
Q

Bifidobacterium

- Normal flora

A

Common fecal flora

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5
Q

Bifidobacterium

- Gram stain

A

Y-shaped GPR

- Non-sporeforming

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6
Q

Lactobacillus

- Normal flora

A

GI and vaginal flora

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7
Q

Lactobacillus

- Gram stain

A

Non-sporeforming GPR

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8
Q

Actinomyces

- Gram stain

A

Often branching GPRs

- Non-sporeforming

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9
Q

Actinomyces israelii

- Colonial morphology

A

ROUGH/SLOW growth

- Molar tooth 5-14 days or longer

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10
Q

Actinomyces israelii

- Gram stain

A

Branching GPRs

  • Non-sporeforming
  • Chunky in broth
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11
Q

Actinomyces israelii

- Diseases or infections it’s involved in

A
  • Actinomycosis → “Lumpy Jaw”

- Sulfur granules from drainage

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12
Q

Mobiluncus

- Gram stain

A

Motile, sligthly curved GPR

- Non-sporeforming

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13
Q

Mobiluncus

- Diseases or infections it’s involved in

A

Non-specific vaginitis or bacterial vaginosis

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14
Q

Eubacterium

- Diseases or infections it’s involved in

A

Rarely cause disease b/c it’s a beneficial bacterium

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15
Q

Eubacterium

- Normal flora

A

Intestinal tract

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16
Q

Eggerthella lenta

- Normal flora

A

Intestinal tract

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17
Q

Three types of spores

A
  • Terminal
  • Central
  • Subterminal
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18
Q

Most clinical isolates of anaerobic gram positive sporeforming rods are ____

A

Clostridium spp

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19
Q

Clostridium perfringens

- Gram stain

A

GPR

  • Sporeforming
  • Often stains Gram negative on a direct smear
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20
Q

Clostridium perfringens

- What are the reactions w/ milk, lecithinase, lipase, and reverse CAMP test?

A
  • Milk: proteolytic (curd/digest)
  • Lecithinase: positive on EYA
  • Lipase: negative on EYA
  • Reverse CAMP: positive
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21
Q

Clostridium perfringens

- Colonial morphology

A

Double zone beta hemolysis

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22
Q

Clostridium perfringens

- 2 major diseases

A
  • Gas gangrene

- Food poisoning

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23
Q

Gas gangrene (myonecrosis)

  • Most common cause
  • Toxins
  • What happens
A
  • Clostriidum
  • Cytotoxins destroy cells/tissues
  • Gas, bullae, discoloration
24
Q

Crepitant (anaerobic) cellulitis

- What is NOT involved?

A

Muscle and facia

25
Clostridium perfringens food poisoning is found in what foods?
Meats and gravy
26
Clostridium ramosum | - Normal flora
GI
27
Clostridium ramosum | - Gram stain
Virgina rail fence and palisading; terminal spores rarely seen
28
Clostridium septicum | - Colonial morphology
Swarms; spreading colonies; slight beta hemolysis
29
Clostridium septicum | - Gram stain
Citron-shaped GPRs
30
Clostridium septicum | - 2 diseases
- Gas gangrene | - Bacteremia associated w/ malignancy (colon cancer, leukemia or lymphoma)
31
Clostridium septicum | - For diseases, are the mortality rates high or low and can therapy make a difference?
- High mortality rates | - Early recognition and proper therapy can make a signficant difference in mortality
32
Clostridium sordellii | - Gram stain
Chaining GPR, w/ lots of free spores
33
Clostridium sordellii | - Colonial morphology
Very irregular colonies
34
Clostridium tetani | - Gram stain
GPRs w/ LARGE terminal spores
35
Clostridium tetani | - Colonial morphology
Irregular shaped colonies, "course ground glass"
36
Tetanus - Another name/what does it cause - Infection or intoxication?
- "Lockjaw" | - Intoxication
37
Clostridium botulinum | - 3 infections
- Foodborne - Infant botulism (aka Floppy Baby Syndrome) - Wound botulism
38
Foodborne C. botulinum - Found in what foods - Infection or intoxication - What occurs from disease - Treatment
- Found in home canned foods - Intoxication - Occurs from descending "flaccid paralysis" - Treatment is an antitoxin
39
Infant botulism caused by C. botulinum - Another name - What food is implicated - Infection or intoxication
- aka Floppy Baby Syndrome - Found in honey - Infection
40
Clostridium sporogenes | - Lipase reaction
Lipase +
41
Clostridium sporogenes | - Gram stain
GPR w/ subterminal and free spores
42
Clostridium sporogenes | - Colonial morphology
Can swarm (filamentous, medusa head colonies)
43
Clostridium difficile | - Normal flora
Intestinal flora
44
Clostridium difficile | - Colonial morphology
Large colonies, irregular edge and shape, raised yellowish to grey-white with a COARSE "CIS"
45
Clostridium difficile | - GLC
GLC = a...IC
46
Clostridium difficile causes an _____ _____ diarrhea
Antibiotic associated → pseudomembraneous colitis
47
What is true about C. difficile diseases's mortality?
Bloody diarrhea can be fatal
48
Why does C. difficile disease potentially serious threat to immunocompromised patients?
Spores on hands, bed sheets, surfaces in hospital environment
49
Clostridium difficile | - 2 major toxins
- Toxin A = enterotoxin | - Toxin B = cytotoxin
50
2 ways to identify Clostridium difficile
- Rapid real-time PCR - Gold standard toxigenic culture for C. diff (using cycloserine, cerfoxitin, fructose agar → CCFA) and ID (but too slow)
51
Rapid real-time PCR assays for which of Clostridium difficile's toxins
Toxin B
52
Clostridium clostridioforme | - Gram stain
GPR that more commonly stains Gram negative; tapered ends | - Football shaped
53
Propionibacterium acnes | - Colonial morphology
May be aerotolerant
54
Aerotolerant Clostridium form spores under ____ conditions
Anaerobic | - Grows better anaerobically compared w/ growth in CO2
55
3 examples of aerotolerant Clostridium
- C. carnis - C. histolyticum - C. tertium
56
Mobiluncus | - Normal flora
Vaginal

Liz's Microbiology Module 2 (11 decks)

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