Anaerobic GNC, GPC, GNRs Flashcards

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1
Q

Most commonly isolated and only GNC implicated as a pathogen

A

Veillonella

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2
Q

Most pathogenic of the anaerobic GPCs and most frequently isolated in pure culture

A

Finegoldia magna

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3
Q

FInegoldia magna

- Colonial morphology

A

Donuts (resembles RBCs)

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4
Q

Parvimonas micra

- Colonial morphology

A

Demonstrates slight beta hemolysis

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5
Q

Peptostreptococcus anaerobius

- Clonial morphology

A

Creamy, round colonies

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6
Q

Veillonella spp

- Clonial morphology

A

Circular, raised, smooth, and creamy

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7
Q

Bacteroids fragilis

- Clonial morphology

A

Fine ground glass (CIS)

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8
Q

Bacteroides ovatus

- Clonial morphology

A

?

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9
Q

Bacteroides thetaiotaomicron

- Clonial morphology

A

Mucoid, often w/ white striations

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10
Q

Campylobacter ureolyticus

- Clonial morphology

A

“Pits” the agar

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11
Q

Prevotella melaninogenica ‘group’

- Clonial morphology

A

Yellow to tan to brown to black colonies → become darker w/ prolonged incubation

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12
Q

Fusbacterium mortiferum

- Clonial morphology

A

Colonies have whirl appearance

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13
Q

Fusobacterium necrophorum

- Clonial morphology

A

“Fried egg” colonies (raised yellow center)

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14
Q

Fusobacterium nucleatum

- Clonial morphology

A
  • Mottled (course ground glass), pinkish to greenish

- Molar tooth forms

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15
Q

Peptostreptococcus anaerobius

- GLC

A

GLC = a…IC

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16
Q

Fusobacterium spp

- GLC

A

GLC = aB

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17
Q

Bacteroides fragilis ‘group’

- GLC

A

GLC = ap (ib) iv

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18
Q

Porphyromonas spp

- Normal flora

A

Mouth

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19
Q

Veillonella spp

- Normal flora

A

Oral, respiratory, intestinal, genitourinary… (mucus membrane lined cavities)

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20
Q

Finegoldia magna

- Normal flora

A

Intestine

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21
Q

Parvimonas micra

- Normal flora

A

Mouth/upper respiratory tract

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22
Q

Staphylococcus saccharolyticus

- Normal flora

A

Skin

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23
Q

Fusobacteirum mortiferum

- Normal flora

A

Colon

24
Q

Fusobacterium nucleatum

- Normal flora

A

Mouth

25
Q

Fusobacteirum necrophorum

- Normal flora

A

Mouth

26
Q

Bacteroides fragilis ‘group’

- Normal flora

A

Colon

27
Q

Prevotella oralis

- Normal flora

A

Mouth

28
Q

Prevotella bivia and P. disiens

- Normal flora

A

Vaginal flora

29
Q

Veillonella spp

- Nitrate reaction

A

Nitrate +

30
Q

Peptococcus niger

- Pigment

A

Black to olive green → pigment fades w/ O2 exposure

31
Q

Finegoldia magna

- Associated w/ what kind of infections?

A

Infections below diaphragm

32
Q

Parvimonas micra

- Associated w/ what kind of infections?

A

Infections above diaphragm

33
Q

Peptostreptococcus anaerobius

- SPS disk test

A

Susceptible ( ≥ 12 mm)

34
Q

Staphylococcus saccharolyticus

- Found in what kind of cultures?

A

Blood and CSF cultures

35
Q

Fusobacterium spp are associated w/ what kind of infections?

A

Infections of the mouth (human bites, lung abscesses, pleural cavity, brain abscess)

36
Q

Fusobacterium nucleatum

- Indole reaction

A

Indole +

37
Q

Fusobacterium necrophorum

  • Associated w what kind of infection?
  • Lipase reaction on EYA
A
  • Common cause of brain abscesses

- Lipase +

38
Q

Fusobacterium mortiferum

  • Associated w/ what kind of infections?
  • Resistant to ____
A
  • Infections below the diaphragm

- Antibiotics

39
Q

Bacteroides fragilis ‘group’

  • Stimulated by what?
  • Bile disk test
A
  • Stimulated by 20% bile

- Bile resistant

40
Q

Bacteroides fragilis ‘group’ is a ____ producer and very resistant antimicrobials

A

Penicillinase

41
Q

Fusobacterium spp

  • Vancomycin disk reaction
  • Kanamycin disk reaction
  • Colistin disk reaction
  • Growth on LKV agar?
A
  • Vancomycin: resistant
  • Kanamycin: susceptible
  • Colistin: susceptible
  • No growth on LKV
42
Q

Bacteroides fragilis ‘group’

  • Vancomycin disk reaction
  • Kanamycin disk reaction
  • Colistin disk reaction
  • Growth on LKV agar?
A
  • Vancomycin: resistant
  • Kanamycin: resistant
  • Colistin: resistant
  • Growth on LKV
43
Q

Bacteroides fragilis ‘group’

- Colonial morphology on BBE agar

A

Large gray-black colonies

44
Q

Bacteroides ovatus

- Indole reaction

A

Indole +

45
Q

Bacteroides thetaiotaomicron

- Indole reaction

A

Indole +

46
Q

Bacteroides fragilis

- Indole reaction

A

Indole –

47
Q

Bacteroides vulgatus

- Indole reaction

A

Indole –

48
Q

Porphymonas spp

- Indole reaction

A

Indole +

49
Q

Porphymonas spp

  • Vancomycin disk reaction
  • Kanamycin disk reaction
  • Colistin disk reaction
  • Growth on LKV agar?
A
  • Vancomycin: susceptible
  • Kanamycin: resistant
  • Colistin: resistant
  • No growth on LKV agar
50
Q

Porphymonas spp

- Associated w/ what kind of infections?

A

Oral dental infections

51
Q

This anaerobe is synergistic (usually found w/ other organisms), requires hemin and vitamin K in vivo, and produces beta lactamase?

A

Prevotella spp

52
Q

Prevotella spp

  • Vancomycin disk reaction
  • Kanamycin disk reaction
  • Colistin disk reaction
  • Growth on LKV agar?
A
  • Vancomyin: resistant
  • Kanamycin: resistant
  • Colistin: variable
  • Growth on LKV agar
53
Q

This anaerobe demonstrates brick red fluorescence and its pigment makes protoporphyrin (causing colonies ot become brown/black)

A

Prevotella melaninogenica

54
Q

Peptostreptococcus anaerobius

- Normal flora

A

Nose, throat, intestine

55
Q

Campylobacter ureolyticus

- Urease reaction

A

Urease +

56
Q

Anaerobic GPCs

A
  • Peptococcus niger
  • Finegoldia magna (large)
  • Parvimonas micra (small)
  • Peptostreptococcus anaerobius (elongated)
  • Staphylococcus saccharolyticus
  • Streptococcus intermedius
57
Q

Anaerobic GNRs

A
  • Fusobacterium spp (spindle-shaped rod → “fusiform”)
  • Fusobacterium nucleatum (fusiform; thin and tapered)
  • Fusobacterium necrophorum (short, long)
  • Fusobacterium mortiferum (HUGE)
  • Bacteroides fragilis
  • Bacteriodes thetaiotaomicron
  • Porphyromonas pp
  • Prevotella spp
  • Prevotella melaninogenic
  • Campylobacter gracilis
  • Campylobacter urealyticus