Anaerobic GNC, GPC, GNRs Flashcards
Most commonly isolated and only GNC implicated as a pathogen
Veillonella
Most pathogenic of the anaerobic GPCs and most frequently isolated in pure culture
Finegoldia magna
FInegoldia magna
- Colonial morphology
Donuts (resembles RBCs)
Parvimonas micra
- Colonial morphology
Demonstrates slight beta hemolysis
Peptostreptococcus anaerobius
- Clonial morphology
Creamy, round colonies
Veillonella spp
- Clonial morphology
Circular, raised, smooth, and creamy
Bacteroids fragilis
- Clonial morphology
Fine ground glass (CIS)
Bacteroides ovatus
- Clonial morphology
?
Bacteroides thetaiotaomicron
- Clonial morphology
Mucoid, often w/ white striations
Campylobacter ureolyticus
- Clonial morphology
“Pits” the agar
Prevotella melaninogenica ‘group’
- Clonial morphology
Yellow to tan to brown to black colonies → become darker w/ prolonged incubation
Fusbacterium mortiferum
- Clonial morphology
Colonies have whirl appearance
Fusobacterium necrophorum
- Clonial morphology
“Fried egg” colonies (raised yellow center)
Fusobacterium nucleatum
- Clonial morphology
- Mottled (course ground glass), pinkish to greenish
- Molar tooth forms
Peptostreptococcus anaerobius
- GLC
GLC = a…IC
Fusobacterium spp
- GLC
GLC = aB
Bacteroides fragilis ‘group’
- GLC
GLC = ap (ib) iv
Porphyromonas spp
- Normal flora
Mouth
Veillonella spp
- Normal flora
Oral, respiratory, intestinal, genitourinary… (mucus membrane lined cavities)
Finegoldia magna
- Normal flora
Intestine
Parvimonas micra
- Normal flora
Mouth/upper respiratory tract
Staphylococcus saccharolyticus
- Normal flora
Skin
Fusobacteirum mortiferum
- Normal flora
Colon
Fusobacterium nucleatum
- Normal flora
Mouth
Fusobacteirum necrophorum
- Normal flora
Mouth
Bacteroides fragilis ‘group’
- Normal flora
Colon
Prevotella oralis
- Normal flora
Mouth
Prevotella bivia and P. disiens
- Normal flora
Vaginal flora
Veillonella spp
- Nitrate reaction
Nitrate +
Peptococcus niger
- Pigment
Black to olive green → pigment fades w/ O2 exposure
Finegoldia magna
- Associated w/ what kind of infections?
Infections below diaphragm
Parvimonas micra
- Associated w/ what kind of infections?
Infections above diaphragm
Peptostreptococcus anaerobius
- SPS disk test
Susceptible ( ≥ 12 mm)
Staphylococcus saccharolyticus
- Found in what kind of cultures?
Blood and CSF cultures
Fusobacterium spp are associated w/ what kind of infections?
Infections of the mouth (human bites, lung abscesses, pleural cavity, brain abscess)
Fusobacterium nucleatum
- Indole reaction
Indole +
Fusobacterium necrophorum
- Associated w what kind of infection?
- Lipase reaction on EYA
- Common cause of brain abscesses
- Lipase +
Fusobacterium mortiferum
- Associated w/ what kind of infections?
- Resistant to ____
- Infections below the diaphragm
- Antibiotics
Bacteroides fragilis ‘group’
- Stimulated by what?
- Bile disk test
- Stimulated by 20% bile
- Bile resistant
Bacteroides fragilis ‘group’ is a ____ producer and very resistant antimicrobials
Penicillinase
Fusobacterium spp
- Vancomycin disk reaction
- Kanamycin disk reaction
- Colistin disk reaction
- Growth on LKV agar?
- Vancomycin: resistant
- Kanamycin: susceptible
- Colistin: susceptible
- No growth on LKV
Bacteroides fragilis ‘group’
- Vancomycin disk reaction
- Kanamycin disk reaction
- Colistin disk reaction
- Growth on LKV agar?
- Vancomycin: resistant
- Kanamycin: resistant
- Colistin: resistant
- Growth on LKV
Bacteroides fragilis ‘group’
- Colonial morphology on BBE agar
Large gray-black colonies
Bacteroides ovatus
- Indole reaction
Indole +
Bacteroides thetaiotaomicron
- Indole reaction
Indole +
Bacteroides fragilis
- Indole reaction
Indole –
Bacteroides vulgatus
- Indole reaction
Indole –
Porphymonas spp
- Indole reaction
Indole +
Porphymonas spp
- Vancomycin disk reaction
- Kanamycin disk reaction
- Colistin disk reaction
- Growth on LKV agar?
- Vancomycin: susceptible
- Kanamycin: resistant
- Colistin: resistant
- No growth on LKV agar
Porphymonas spp
- Associated w/ what kind of infections?
Oral dental infections
This anaerobe is synergistic (usually found w/ other organisms), requires hemin and vitamin K in vivo, and produces beta lactamase?
Prevotella spp
Prevotella spp
- Vancomycin disk reaction
- Kanamycin disk reaction
- Colistin disk reaction
- Growth on LKV agar?
- Vancomyin: resistant
- Kanamycin: resistant
- Colistin: variable
- Growth on LKV agar
This anaerobe demonstrates brick red fluorescence and its pigment makes protoporphyrin (causing colonies ot become brown/black)
Prevotella melaninogenica
Peptostreptococcus anaerobius
- Normal flora
Nose, throat, intestine
Campylobacter ureolyticus
- Urease reaction
Urease +
Anaerobic GPCs
- Peptococcus niger
- Finegoldia magna (large)
- Parvimonas micra (small)
- Peptostreptococcus anaerobius (elongated)
- Staphylococcus saccharolyticus
- Streptococcus intermedius
Anaerobic GNRs
- Fusobacterium spp (spindle-shaped rod → “fusiform”)
- Fusobacterium nucleatum (fusiform; thin and tapered)
- Fusobacterium necrophorum (short, long)
- Fusobacterium mortiferum (HUGE)
- Bacteroides fragilis
- Bacteriodes thetaiotaomicron
- Porphyromonas pp
- Prevotella spp
- Prevotella melaninogenic
- Campylobacter gracilis
- Campylobacter urealyticus
