Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > Anaerobe Lecture pt 1 > Flashcards

Anaerobe Lecture pt 1 Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

How much oxygen is in each kind of air/system for the following?

  • ambient air
  • CO2 incubator
  • microaerophilic system
  • anaerobic system
A
  • ambient air: 21%
  • CO2 incubator: 15%
  • microaerophilic system: 5%
  • anaerobic system: 0%
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what is a strict or obligate aerobe?

A

like oxygen and use O2 as a terminal electron acceptor during metabolism (metabolism during respiration)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

how do facultative anaerobes grow in the presence and absence of oxygen?

A

presence: grow using aerobic respiration

absence: fermentation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what are aerotolerant anaerobes?

A

grow poorly in ambient air (21% oxygen) and better in anaerobic conditions

use fermentation for energy and growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

microaerophilic organisms are capable of growing in what percentage of oxygen? What are two examples of microaerophilic organisms?

A

5%

  • H. pylori and Campy spp.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

moderately obligate anaerobes tolerate oxygen at what percentages? What are two examples?

A

2-8%
- Bacteroides and many Clostridium spp.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

strict obligate anaerobes cannot tolerate more than what percent of oxygen?

A

0.5%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

normal human tissue has a high or low redox potential? What is meant by this?

A

high

it mean that they have a high amount of oxygen reactions happening, which don’t make for hospitable anaerobe environments

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

infected human tissue have what kind of redox potential that make them a good environment for anaerobes?

A

low redox potential

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what are three common reducing agents in anaerobic media?

A
  • thioglycolate
  • cysteine
  • dithiothreitol
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

in the colon, anaerobic bacteria outnumber enterobacterales in what ratio?

A

1000 to 1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

what are four popular anaerobic habitats?

A

water, soil, some animals, and humans (URT, GI / intestinal tracts, and oral cavity)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what are manifestations of anaerobe infections?

A
  • necrotic tissue
  • foul-smelling discharge
  • gaseous discharge
  • black exudate
  • sulfur granules
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what are acceptable specimens for anaerobes?

A

tissue/biopsy material, aspirates, normally sterile body fluids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what color is the oxygen indicator Resazurin when anaerobic and aerobic conditions?

what about methylene blue when exposed to O2?

A

Resazurin:
anaerobic: colorless
aerobic: pink

Methylene blue: blue when exposed to oxygen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

what kind of loops can you not use when processing specimens for anaerobic growth?

A

nichrome loops because they oxidize the inoculum and culture media

17
Q

what does PRAS stand for?

A

pre-reduced anaerobically sterilized

18
Q

what is the one type of nonselective anaerobic media and the types of this media?

A

anaerobic blood agar (anaBAP)
- CDC anaerobic blood
- brucella blood
- enriched BHI
- Schaedlers

19
Q

what is an anaPEA and CCFA and their functions as anaerobic media?

A

anaerobic phenylethyl alcoholic blood agar:
- inhibits enterobacterales
- supports GPC and anaerobes

Cycloserine-cefoxitin fructose agar
- selective, differential, and inhibitory
- used for specifically C. diff

20
Q

what is a BBE agar?

A

bacteroides bile esculin agar
- selective and differential
- isolates and presumptive ID of B. fragilis spp.

21
Q

what three things does the BBE agar contain?

A

gentamicin (an antibiotic), bile, and esculin

22
Q

KVLB agars are both ______ and ______. What two spp will grow on it?

A

Kanamycin-vancomycin laked blood agar
- selective and inhibitory
- growth for specifically Bacteroides and Prevotella spp.

23
Q

what is the anaerobic broth used in the micro lab? What must be done to it prior to use?

A

Thioglycollate (thio) broth must be boiled (reduced) before usage

24
Q

what are the kinds of gas used to incubate anaerobes?

A

nitrogen (inactive), hydrogen (active), and carbon dioxide (for capnophiles like Campy)

25
Q

what is the catalyst in anaerobic jars?

A

palladium

26
Q

what is the aerotolerance test?

A

to see if a colony is facultative or strict anaerobe:
- incubate colony on both chocolate (grow in aerobically) and anaBAP (grow anaerobically)

Microbiology (13 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions