Ampelography - T. Lacombe + Others Flashcards
Why is ampelography important (4 reasons)
-describe* & identify grapevine
-origin & evolution of varieties
-behavior & potential of varieties
-preservation of genetic resources
A minor variation in a vine = _________, a major variation =_________
clone
new cultivar
Cultivar
AKA variety, cepage, vitigno
-cultivated variety
-initially produced from one seed, then vegetatively propegated
-variable number of clones, variations can occur
Clone
AKA biotype, selection, type
Definition according to OIV: A clone is the vegetative progeny of a single vine plant. For selection purposes this single plant is chosen for its varietal identity, its phenotypic traits and its sanitary state.
Synonym versus homonymy
synonym: serveral names for one cultivar
homonymy: one name for several cultivars (riesling, grey riesling, emerald riesling)
Teinturier
“ten-tur-ee-aye”
grapes with colored flesh
Methods of ampelography identification
-Morphology (phenotype) = Ampelography, Ampelometry, Image analysis, Phenology (bud break, veraison, maturity, etc), Palynology/caryology (pollen study)
-Chemical taxonomy
-Biochemical markers
-Molecular markers (genotype)
Two types of hairiness on vines
- Prostrate (or wooly, cotton) hairs
- Erect (or pubescent) hairs
When is it best to extract DNA, and when is it not
-best on young leaves, but can be done on other vine parts and juice.
-very challenging to be done on wine
Molecular marker: SSRs
-Extraction of DNA
-Simple Sequence Repeats = Microsatellites
DNA written in four letter code, repitition of letters counted
Biochemical markers: the isoenzymes
-polymorphism between enzymes and catalyst
=when given a certain catalyst, each variety gave specific markers, a chart was created
Molecular marker: PCR
-extraction of DNA
-chain amplification
-Polymerase Chain Reaction
Simple Nucleotide Polymorphism = SNP
-change in a single base of a microsatellite sequence
What are the two main tests used in molecular markers and what are the differences between them?
SSR: Simple Sequence Repeats = Microsatellites
-identifies varieties only
-can only test vine organs (trunk included) or juice
-referencr chart used to identify varieties
SNP: Single Nucleotide Polymorphism
-identifies varieties and clones
-can test vine organs, wine and ancient DNA (from old jugs/bottles)
Eukaryot versus prokaryot cells
-Eukaryotic cells are cells containing membrane-bound organelles and are the basis for both unicellular and multicellular organisms (animals and plants)
-prokaryotic cells do not have any membrane-bound organelles and are always part of unicellular organisms (bacteria)
How many genus does the Vitaceae have
21; Vitis, Ampelocissus, Cissus, Parthenocissus (japanese ivy), etc
How many diferent species does Vitis have
70; Vitis vinifera, Vitis rupestris, etc
Botanical characteristics of Vitis
- Shrubs with perennial stocks
- Tendrils without suction grip
- Inflorescences in cluster, opposite to leaves
- Flowers with 5 base units
- Calyptra anthesis
- Free stamens
- Ovary on top
Differences between Vitis and Muscadinia
- Number of chromosomes n=19, n=20
- Position of the subero‐phelodermic layer
- Bark
- Pith and xylem of the shoots
- Division of the tendrils
- Shape of the seeds
How many American Vitis species
21 species (+ 4 natural hybrids)
-classified into 6 series
How many Asian Vitis species
54 species and counting
-not regularly updated