Amino Acids, Dna And Proteins Flashcards
Amino acid
Simple organic compound containing a carboxyl and an amino group.
Why are amino acids optically active
They have a single chiral centre
Zwitterion
A molecule or ion having separate positively and negatively charged groups
Neutral overall charge
Amphoteric properties of amino acids
Carboxyl group can lose a proton and the amine group is able to accept a proton.
Isoelectric point
The pH at which the amino acid does not migrate in an electric field. At neutral pH, the Zwitterion form of the amino acid is dominant.
Properties of amino acids
They are ionic and so have high melting points and are soluble in polar solvents.
White solids at room temperature.
In strong acidic conditions, the lone pair of the H2N- group accepts a proton to form a positive ion/become proponated.
The OH- group loses
How do you set up electrophoresis ?
Moistened filter paper on a microscope slide with a crocodile clip at each end attached to a battery.
A drop of amino acid solution is placed in the centre of the paper.
Spray with ninhydrin to see position on paper.
Expected result of electrophoresis with negative amino acid?
Would travel towards anode
Give a use of CH3(CH2)17COO- Na+
Soap
Give a use of CH3(CH2)19COOCH3
Biofuel
Give a use of [C16H33N(CH3)3]+ Br-
Detergent
Shampoo
Conditioner
Fabric softener