Allergies Flashcards
- Describe the components of immediate hypersensitivity reactions.
antigen from the environment is processed by an antigen presenting cell APC, a TH2 response dominates which drives IgE , IgE binds to receptors on mast cells and basophils
IgE on mast cells results in release of mediators the produce end organ response
- Identify the major difference between mast cells and basophils (location, histamine content, steroid effects, cyclooxygenase inhibition)
Mast: located in tissues and contain much more histamine, minimal steroid effects, no effect from cylcooxygenase pathway inhibition (immediate-predominate rxn.)
basophils: in the peripheral blood, steroids inhibit mediator release, and COO pathway inhibition augments mediator release, lesser histamine content (late-predominant reaction
- Describe the mechanisms and agents responsible for mast cell activation/secretion.
cross linking of IgE receptors is essential for mast cell/basophil activation (allergen binds at least two IgE molecules)
secretion can be triggered by IgE, endogenous stimulus (complement, neuropeptides, ATP, adenosine, cytokines etc.) and exogenous factors (anesthetic agents, antibiotics, narcotics and iodinated contrast material)
- List the principal preformed and generated mast cell mediators.
preformed: vasoactive amines (histamine, serotonin), proteoglycans (heparin), neutral proteases (tryptase, chymotrypitc proteases), acid hydrolyses (B hyxosaminidase and B glucoronidase, chemotactic factors for eos and polys and peroxdase/superoxide
- Describe late-phase reactions.
over hours cytokines are produced and released, speficially IL-4, IL-13
- Compare the skin test and serum IgE procedures for sensitivity testing.
allergen hypersensitivity on skin- detects IgE on skin mast cells (most common and most sensitive test used clinically)
immunoassays for specific Ig (ie. RAST or ELISA)
Provocation studies “challenge”- tests tolerance
basophil/mast cell histamine release- tests activation
- Describe the signs and symptoms of allergic rhinitis.
congestion, post nasal drip and rhinorrhea, pruritus, sneezing, ocular congestion, redness, itching
complications: sinusitis, nasal polyposis, otitis media and allergic conjunctivitis
- Describe the pathogenesis of allergic rhinitis. (immediate and late phase)
binding of allergen to allergen specific IgE on mast cells causes mediator release (preformed: histamine and proteases; De novo: arachidonic acid metabolites and cytokines)
late phase inflammation involves increased expression of cell adhesion molecules by endothelium, local release of cytokines and chemotactic factors and eosinophils, basophils and activated lymphocytes
- Discuss the methods to diagnose allergic rhinitis.
hx: seasonal or perennial pattern of allergen
PE: swelling of nasal mucosa, conjunctival inflammation, allergic shiners, Dennies lines, nasal crease, mouth breathing
dx. tests (when needed): Serum IgE, skin tests,
- Discuss the different modes of therapy for allergic rhinitis.
environmental control measures
mediator antagonists (antihistamines, topical anticholinergics, leukotriene inhibitors and antagonists, decongestants)
anti-inflammatory agents
mast stabilizers
immunotherapy
- Describe the pathogenesis of allergic asthma.
TH2 shift magnifies sensitization and allergic response leading to airway inflammation and eventually airway remodeling
- Define the following terms: atopy and allergen.
allergen is an antigen capable of eliciting an IgE antibody response
Atopy- a genetic predisposition to the formation of increased levels of IgE antibody (factors that play a role: changes in allergen levels, increased environmental pollution, dietary changes, alterations in exposure to microbial pathogens)
IL-4, IL-13 will increase IgE production while INF gama will decrease IgE
- Differentiate food allergy and food intolerance.
allergies have immunological mechanism
food intolerance is due to non-immunologic cause (ie. metabolic, food poisoning, pharmacologic etc. )
- Discuss the clinical manifestations of food allergy.
cutaneous manifestations (hives, pruritus, eczema)
GI (itching, nausea, vomiting diarrhea, abdominal pain)
respiratory (rhinoconjuctivitis, wheezing, hoarseness)
anaphylaxis
neurologic manifestation (feeling of dread)
- Differentiate drug allergy from other adverse reactions to drugs.
food allergies are reproducible, and are eliminated if food is eliminated
drug reactions can be predictable (occur in most normal individuals) or unpredictable (occurring in a small subset of the population- idiosyncratic or immunologic)