Agar and Organism Flashcards
Bile esculin agar
Enterococcus
Blood agar: α-hemolytic
Strep. Pneumo, Viridans Strep, Enterococcus
Blood agar: β-hemolytic
Ton’s of things: S. aureus, S. pyogenes, S. agalactia, Staph lugdunensis(coagulase negative and B-hemolytic makes it unique)
Blood agar: γ-hemolytic
Enterococcus
Blood agar: CAMP positive
Strep. Agalactiae, Listeria
Bordet-Gengou agar
B. pertussis
Buffered charcoal yeast extract agar
Legionella
Chocolate agar – with factors V and X
H. influenza
Chocolate agar – with factors X only
H. ducreyi
Lowenstein-Jensen agar
M. tuberculosis
MacConkey agar
E. coli and K. pneumonia
Regan-Lowe media
B. pertussis
Sabouraud dextrose agar
Fungi
Sorbitol MacConkey agar
All E.coli, except EHEC can’t ferment sorbitol
Thayer-Martin agar
N. gonorrhoeae or meningitides
Thiosulfate citrate bile salts sucrose agar
Vibrio
Eaton medium
M. pneumonia
Egg yolk agar
Clostridium
Eosin Methylene Blue (EMB) agar
E. coli – grows metallic green colonies
Hektoen enteric agar
Salmonella and Shigella
Loeffler media
C. diphtheria
Mannitol salt agar
S. aureus
Tellurite media
C. diphtheria
Thioglycolate broth
Depends on where the growth in the broth takes place. If only at top = aerobe. Only at bottom = anaerobe. If there is growth diffusely throughout the tube = facultative anaerobe
Triple Sugar Iron (TSI) agar
If the organism produces hydrogen sulfide (Salmonella or Proteus) then agar turns black
Xylose-Lysine-Deoxycholate (XLD) agar
Colonies with black centers = Salmonella.
Shigella colonies are red.
