Advanced Tumor Suppressor Genetics

Question 1

Senescence-associated secretory phenotype

Answer 1

The phenotype produced by the cellular regulators of senescence.

Involves the secretion of immunoactive cytokines, chemokines, matrix remodeling enzymes, and growth factors, inculding CXCL14, IL-7, and IL-34.

CXCL14 helps recruit macrophages while IL-7 and IL-34 induce MO to M1 polarization. Once macrophages are M1 polarized, they go on to recruit CD8 T cells.

Question 2

Cellular markers of senescence

Answer 2

SA-beta-galactosidase

p16

lack of Ki67

Remember, quiesence is maintained by the Cip/Kip family!

Question 3

CXCL14

Answer 3

Chemokine up-regulated in a p21-locked senescent state that recruits macrophages

Question 4

p21-mediated immunosurveillence

Answer 4