Acts

Question 1

CLOT RETRACTION TIME:

1. Within ____ after whole blood is allowed to clot in a clean glass tube at 37C, the clot will begin to shrink and retract from the walls of the tube
2. _____ is expressed and clot becomes denser
3. Specimen needed
4. Procedure Method
5. Factors that affect clot retraction

Answer 1

1. 1hr
2. SERUM
3. Fresh whole blood
4. Modified MacFarlane Serum method
5. 13a, Fibrinogen, Thrombin

Question 2

CLOT RETRACTION TIME: Procedure

1. Collect ___mL of venous blood samples
2. Incubate for ____hours
3. Serum expressed is centrifuged for _____mins at ____rpm
4. Volume of serum formula
5. % serum expressed formula
6. Reference value

Answer 2

1. 5mL
2. 2hrs
3. 3-5 minutes at 3,500 rpm
4. total volume of serum in tube – volume of packed red cells
5. (Volume of Serum / Volume of blood used in mL) x 100
6. 44-67

Question 3

WHOLE BLOOD COAGULATION TIME:

1. This measures the overall ___ and ____ pathways of coagulation
2. Micro method
   - reference value
3. Macro method
   - reference value

Answer 3

1. Intrinsic and Common
2. Slide
   - 2-4mins
3. Lee & White
   - 7-15mins

Question 4

APTT & PT:

1. useful procedure for routine screening of coagulation disorders in the intrinsic and common pathways
   - PPP contains all coagulation factors needed for generation of intrinsic _____ except _____ and _____
   - when calcium is added with incomplete thromboplastin, intrinsic _____ is generated
   - _____ is converted to ____ which cleaves fibrinogen intro fibrin clot
2. useful screening procedure for the extrinsic and common pathways of coagulation.
   - when _____ or ____ is added to PPP along with calcium
   - it reacts with factor ___ to convert factor ____ to its activated from which initiates common pathways

Answer 4

1. APTT
   - prothrombinase/plasma thromboplastin except calcium & platelet phospholipid
   - intrinsic prothrombinase
   - prothrombin is converted to thrombin
2. PT
   - tissue extract or thromboplastin
   - 7; 10

Question 5

APTT & PT

1. Specimen Required
2. Procedure method
3. Expected PT
4. Expected APTT

Answer 5

1. Citrated plasma
2. Tilt Tube Technique (Manual)
3. 12-15
4. 22-34

Question 6

PT:

1. Procedure Reference
2. Pre incubate the reconstituted PT reagent for _____
3. Pipette ____uL test plasma for 1min
4. Add ____uL of preincubated Pt reagent

Answer 6

1. QuickCoag
2. 10mins
3. 100uL
4. 200uL

Question 7

APPT:

1. Procedure Reference
2. Pre incubate the Calcium Chloride for _____
3. Pipette ____uL test plasma for 1-2mins
4. Add ____uL of APTT reagent
   - then incubate for ____
5. Add ____ of pre incubated calcium chloride

Answer 7

1. AMS COMPANY
2. 10mins
3. 100uL
4. 100uL
   - 3mins
5. 100uL