Activity 5 - Determination of Proteins in Urine - AUBF LAB Flashcards
Normally, urine contains __ - __ mg/dL of protein per 24 hours
1 - 14 mg/dL
Proteins in normal urine mostly consist of ____ and ____
plasma albumin, globulins
Other proteins that originate from RTE
It is also known as Tamm-Horsfall. A mucoprotein synthesized by distal tubular cells
Uromodulin
Other proteins that originate from RTE
A fibrolytic enzyme
Urokinase
Other proteins that originate from RTE
Secretory Immunoglobulin __?
A
For most patient with proteinuria, ___ is the protein present in increased concentration
Albumin
____ and ____ screening test for urine protein rely on precipitation techniques.
Semiquantitative and Qualitative
Robert’s reagent is composed of ___ and ___
magnesium sulfate and nitric acid
Robert’s Test
Obeserve formation of white ring at the zone of contact of the two liquids within __ - __ minutes
2 - 3 minutes
Heller’s reagent is composed of ___
pure nitric acid
Heller’s Test
Obeserve formation of white ring at the zone of contact of the two liquids within __ minutes
2 minutes
Heller’s Test
If the ring appears only after __ minutes, then the amount of albumin is <_____%
0.0005%
Albumin is precipitated by heat and coagulated by acetic acid. The amount of precipitate or cloudiness formed is directly proportional to the amount of albumin present. What type of test is this?
Salt and Acetic Acid Test
SAA Test
Centrifuge 10 mL of fresh urine specimen at __ rpm for __ - __ minutes
60 rpm, 10-15 minutes
SAA test
The salt holds the ___ in the solution and the acid dissolves the ___ so that any clouding may be considered to indicate the presence of albumin or globulin.
nucleoprotein, mucin
SAA Test
Result:
no cloudiness or no increase in turbidity
Negative
SAA Test
Result:
faint turbidity; diffuse cloudiness is observed against a black background
Positive (trace)
SAA Test
Result:
small amount of precipitate, < 1/4 of the solution
1+
SAA Test
Result:
precipitate is 1/4 - 1/2 of the solution
2+
SAA Test
Result:
precipitate is 1/2 - 3/4 of the solution
3+
SAA Test
Result:
precipitate is > 3/4 of the solution
4+
This test is based on cold precipitation of urine protein with a strong acid
Sulfosalicylic Acid Test for Urine Protein
SAA Test
How many ml of 7% sulfosalicylic acid reagent should be added?
7.5 mL
SAA Test
egati
Result:
No turbidity, or no increase in turbidity
Negative
SSA Test
Result:
Barely visible turbidity
Trace
SSA Test
Result:
No turbidity, or no increase in turbidity
Negative
SSA Test
Result:
Distinct turbidity
1+
SSA Test
Result:
Moderate turbidity
2+
SSA Test
Result:
Heavy turbidity but no flocculation
3+
SSA Test
Result:
Heavy and flocculent turbidity
4+
_____ is a low molecular weight protein that is excreted by persons with proliferative disorder like multiple myeloma
Bence Jones Protein
_____ is the method of choice for diagnosing suspected cases of multiple myeloma to confirm screening methods by urinalysis
Serum electrophoresis
Most of the screening tests are based on precipitation of BJP at temperature between __ - __C and solubility at ___C
40-60, 100
Clinical proteinuria is indicated at less than or equal __mg/dL
30 mg/dL
Esbach’s Test
Proteins in urine even in the smallest (trace) amount react with and are precipitated by ___. The amount of precipitated is measured in g/L
picric acid
