Activity 5 - Determination of Proteins in Urine - AUBF LAB

Question 1

Normally, urine contains __ - __ mg/dL of protein per 24 hours

Answer 1

1 - 14 mg/dL

Question 2

Proteins in normal urine mostly consist of ____ and ____

Answer 2

plasma albumin, globulins

Question 3

Other proteins that originate from RTE

It is also known as Tamm-Horsfall. A mucoprotein synthesized by distal tubular cells

Answer 3

Uromodulin

Question 4

Other proteins that originate from RTE

A fibrolytic enzyme

Answer 4

Urokinase

Question 5

Other proteins that originate from RTE

Secretory Immunoglobulin __?

Answer 5

A

Question 6

For most patient with proteinuria, ___ is the protein present in increased concentration

Answer 6

Albumin

Question 7

____ and ____ screening test for urine protein rely on precipitation techniques.

Answer 7

Semiquantitative and Qualitative

Question 8

Robert’s reagent is composed of ___ and ___

Answer 8

magnesium sulfate and nitric acid

Question 9

Robert’s Test

Obeserve formation of white ring at the zone of contact of the two liquids within __ - __ minutes

Answer 9

2 - 3 minutes

Question 10

Heller’s reagent is composed of ___

Answer 10

pure nitric acid

Question 11

Heller’s Test

Obeserve formation of white ring at the zone of contact of the two liquids within __ minutes

Answer 11

2 minutes

Question 12

Heller’s Test

If the ring appears only after __ minutes, then the amount of albumin is <_____%

Answer 12

0.0005%

Question 13

Albumin is precipitated by heat and coagulated by acetic acid. The amount of precipitate or cloudiness formed is directly proportional to the amount of albumin present. What type of test is this?

Answer 13

Salt and Acetic Acid Test

Question 14

SAA Test

Centrifuge 10 mL of fresh urine specimen at __ rpm for __ - __ minutes

Answer 14

60 rpm, 10-15 minutes

Question 15

SAA test

The salt holds the ___ in the solution and the acid dissolves the ___ so that any clouding may be considered to indicate the presence of albumin or globulin.

Answer 15

nucleoprotein, mucin

Question 16

SAA Test

Result:

no cloudiness or no increase in turbidity

Answer 16

Negative

Question 17

SAA Test

Result:

faint turbidity; diffuse cloudiness is observed against a black background

Answer 17

Positive (trace)

Question 18

SAA Test

Result:

small amount of precipitate, < 1/4 of the solution

Answer 18

1+

Question 19

SAA Test

Result:

precipitate is 1/4 - 1/2 of the solution

Answer 19

2+

Question 20

SAA Test

Result:

precipitate is 1/2 - 3/4 of the solution

Answer 20

3+

Question 21

SAA Test

Result:

precipitate is > 3/4 of the solution

Answer 21

4+

Question 22

This test is based on cold precipitation of urine protein with a strong acid

Answer 22

Sulfosalicylic Acid Test for Urine Protein

Question 23

SAA Test

How many ml of 7% sulfosalicylic acid reagent should be added?

Answer 23

7.5 mL

Question 24

SAA Test
egati
Result:

No turbidity, or no increase in turbidity

Answer 24

Negative

Question 25

SSA Test

Result:

Barely visible turbidity

Answer 25

Trace

Question 26

SSA Test

Result:

No turbidity, or no increase in turbidity

Answer 26

Negative

Question 27

SSA Test

Result:

Distinct turbidity

Answer 27

1+

Question 28

SSA Test

Result:

Moderate turbidity

Answer 28

2+

Question 29

SSA Test

Result:

Heavy turbidity but no flocculation

Answer 29

3+

Question 30

SSA Test

Result:

Heavy and flocculent turbidity

Answer 30

4+

Question 31

_____ is a low molecular weight protein that is excreted by persons with proliferative disorder like multiple myeloma

Answer 31

Bence Jones Protein

Question 32

_____ is the method of choice for diagnosing suspected cases of multiple myeloma to confirm screening methods by urinalysis

Answer 32

Serum electrophoresis

Question 33

Most of the screening tests are based on precipitation of BJP at temperature between __ - __C and solubility at ___C

Answer 33

40-60, 100

Question 34

Clinical proteinuria is indicated at less than or equal __mg/dL

Answer 34

30 mg/dL

Question 35

Esbach’s Test

Proteins in urine even in the smallest (trace) amount react with and are precipitated by ___. The amount of precipitated is measured in g/L

Answer 35

picric acid