A: topic 5 and 6 Flashcards
plant cell specifications
Cell wall – plasmodesmata form a communication between the plant cells
Plastids
Vacuoles
Inclusions
Ploidy
Ploidy level: Number of chromosome sets in a cell
Polyploid: more than two sets (haploid, diploid)
Triploid species: bigger vegetative body, better resistance
Tetraploid species: increased production of reproductive organs Goal of breeding: better attribution
vacoules
- how are they made and of what
- different types
- tonoplast membrane storing solid or fluid compounds and gases
Vesicles of ER and dictyosomes(many together form the gogi apparatus) -> provacuoles
The provacuoles fuse into larger ones -> central vacuole
(In animal cells the vacuoles are of little importance)
- Storage(isolating metabolites), cell lysis, maintaining hydrostatic pressure and pH, dispertion and protection
lysosomes
Thick membrane
Hydrolytic enzymes in the middle – autophagy
vegetative (storage) vacoules
Diluted water solution, mildly acidic pH 5-6 (BUT pl. lemon pH 2)
Ions, by-products, waste products, nutrient storage
inclusions
No constant, but not essential substances
No metabolic activity
no membranes
Protein inclusions; globoid-, crystalloid forms, aleurone layer
Carbohydrate inclusion; mainly stach
oil inclusions: oil droplets
crystals
Accumulation of inorganic compounds: Oxalates, carbonates
silicates Digestion problems, irritation
plastids
1. fuctions and types
1.
Assimilation, synthesising metabolic processes, storage functions
Coloured plastids: chloro- and chromoplastids
Colourless plastids: leukoplastids
- Plastid development
Originates from prokaryotes
Proplastid
No sunlight: Etioplastids(an example of leukoplastids)
Sunlight: chromoplastids
Leucoplastid
Colourless plastids in organs not exposed to sunlight
Storage function!
The plant uses the stored nutrients or plastids can turn into green ones (potato)
Globular or fusiform shape
Examples:
Proteinoplastids – protein
Simple or compound types
storing and modifying proteins
In modified stems, seeds
Chromoplastids
Originated from proplastids or amiloplastids or chloroplastids (ripening, aging leaves)
Less developed inner membranes
Carotenoid pigments
carotene, xanthophylls: the two classes of carotenoid pigments
autumn leaf pigment
Globular, fibrillar or chrystalline forms
In flowers, fruits, leaves, roots Pollination, dispersal
Chloroplast
- Structure and function of thylakoid
- Compounds and functions of stroma
- plastid for C4 plants
1.
Outer membrane similar to cell membrane
Photosystems: intergal proteins and pigments(chlorophyll on membrane)
Electron transport chain: proteins ATP and NADPH production -> photosynthesis (light dependent reactions)
2. RuBisCO enzyme (protein complex)
Photosynthesis (light independent reaction, calvin) – production of glucose and intermediate products
3.
For C4 plants mesophyll cells has a granum structure, but it’s absent from the bundle-sheath cells!
Mesophyll cell and bundle sheath cell are much more closely related: unique step first in mesophyll cell, where CO2 and PEP is the first step to making malate, which will diffuse into the bundle sheath cell where the calvin cycle will happen as normal just with malate as a starting product instead of CO2. glucose is made
the first step in the mesophyll cell is there because PEP can only use CO2 even in low concentrations, while rubisco would have used O2 instead. this way the O2 is also separated form the calvin cycle so that only CO2 is used even in high temp., drought, low CO2
cell wall: major functions - 6
Protection (environmental stress, pathogenes, water loss)
Gives a definite shape
Enables information transport to the exterior
Helps in osmotic-regulation, prevents water loss
Transport processes
ion exchange, mineral uptake (root hairs)
what is the “skeletal structure” of the cell wall
The cellulose:
1,4 linked β–D glucose chain
- primary cell wall
- secondary cell wall
- what can decompose the cell wall?
1.
Primary cell wall:
web of microfibrils + auxin ->loosened structure, formation of new fibrils -> increasing cell wall surface (the “original”, basic cell wall)
- Secondary:
new transverse layers of cellulose fibrils (increased strenght) (layers on top of the primary wall – like padding a brick wall, more secondary wall, decreasing digestibility)
Outer (centrifugal) thickening In case of spores and pollens Spikes, grains, appendages…
Inner thickening: strengthening and protection
3.
Cellulase enzymes ->decomposition of cell wall (secretory ducts, transport vessels)
matrix substances of the cell wall: 3
Pectins (soluble fiber)
- high proportion in fruits and storage organs
Hemicellulose
- cross-link between pectins and cellulose (e.g.: arabinoxylan)
Proteins
- soluble (hydrolitic enzymes, peroxidases)
- insoluble (=extensins) – web-like structure
incrustations of the cell wall: 3
Pigments, Mucilage
- storage of water, gelling effect, Inorganic compounds – in plant hairs -> irritation
Lignin (wood)
- phenolic polymers consists of coumaryl alcohol, coniferyl alcohol, sinapyl alcohol Mainly in the secondary wall (lignification) Increase rigidity Decrease digestibility!
Suberin (cork)
- polymers of aromatic and aliphatic compounds Waterproof layer, decreasing evaporation Decreasing digestibility!
adcrustations
where, what: 2
On cell wall surface
lipid coating: Wax or cutin
Water insulators: decreasing evaporation
plasmodesmata
Channels traverse the cell walls – smooth ER tubes and protein filaments Direct transport between cells
Large number in young cells, later decreasing abundance