9.6 RBC freezing Flashcards

1
Q

RBC freezing is a long term storage for how many years?

A

10

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2
Q

When RBC is thawed and washed, what is the expiration ?

A

24 hours

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3
Q

The main purpose of this is to prevent lysis of red cells during freezing?

A

Cryoprotective agent

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4
Q

2 types of cryoprotective agents

A

Penetrating and nonpenetrating agent

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5
Q

Type of cyroprotective agent that is a small molecules that cross to the cell membrane into the cytoplasm

A

Penetrating agent

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6
Q

Example of penetrating agent?

A

Glycerol

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7
Q

Type of cyroprotective agent that that is a large molecules that do not enter the cell instead, forms a shell around it

A

Non penetrating agent

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8
Q

Example of nonpenetrating agent?

A

Hydroxyethyl starch

Dimethylsulfoxide

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9
Q

In high glycerol:

What is the initial freezing temp?

A

-80C

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10
Q

In low glycerol:

What is the initial freezing temp?

A

-196C

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11
Q

In high glycerol:

What is the type of freezer?

A

mechanical

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12
Q

In low glycerol:

What is the type of freezer?

A

Liquid nitrogen

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13
Q

In high glycerol:

What is the Maximum storage temp?

A

-65C

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14
Q

In low glycerol:

What is the Maximum storage temp?

A

-120C

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15
Q

In high glycerol:

What is the Shipping requirements?

A

Dry ice

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16
Q

In low glycerol:

What is the Shipping requirements?

A

Liquid nitrogen

17
Q

In high glycerol:

What is the expiration ?

A

10 years

18
Q

Low glycerol is commonly used

T or F

A

F

High

19
Q

Process of removing glycerol in the RBC

A

Deglycerolization

20
Q

What is the process of deglycerolization?

A

Decrease saline concentration

21
Q

Decrease saline is from isotonic to hypotonic

T or F

A

F

Hyper to iso

22
Q

What is the 1st decrease saline concentration

A

12%

23
Q

What is the 2nd decrease saline concentration

A

1.6%

24
Q

What is the 3rd decrease saline concentration

A

0.09% W/ 0.2% dextrose

25
Q

Donor with Sickle cell Trait _____ omitted because it will _____ the cell

A

1.6%

Lyse

26
Q

Automated Removal of glycerol is Close system

T or F

A

T

27
Q

What is the expiration date for Automated removal of glycerol?

A

14 days at 4 C

28
Q

Expiration for Automated removal of glycerol is 14 days at 4C that is suspended at AS-_

A

3