9.1 Blood Component PReparation Flashcards
Use of Transfusion therapy:
Inadequate Oxygen carrying capacity because of _________________
Insufficient coagulation proteins or platelets to provide adequate hemostasis
Anemia or Blood loss
2 types of Blood Collection:
Collection of Blood in a sterile container
Close system
2 types of Blood Collection:
Collection or exposure to air through an open port that would shorten the expiration because of potential bacterial contamination
Open system
What is the expiration for open system RBC?
24 hours
What is the expiration for open-system platelets
4 hours
Platelet concentrate is stored at what temp?
Room temp
The Goal of RBC Preservation is to provide viable and functional blood components for patients requiring blood transfusion
T or F
T
FDA requires an average of ____ hour post transfusion for RBC preservation which allows RBC survival of more than ____%
24, 75
For RBC Preservative Anticoagulant:
What is the Storage time for:
Acid-Citate-Dextrose (ACD)
21 days
For RBC Preservative Anticoagulant:
What is the Storage time for:
Citrate-Phosphat-Dextrose (CPD)
21 Days
For RBC Preservative Anticoagulant:
What is the Storage time for:
Citrate-Phosphate-Double-Dextrose (CP2D)
21
For RBC Preservative Anticoagulant:
What is the Storage time for:
Citrate-Phosphate-Adenine (CPDA-1 and 2)
35
RBC preservative that Allows RBC to store for 10 years
Frozen RBC
Benefits of RBC Additive Solutions:
Extends the Shelf-life of RBC to __ days by adding nutrients
42
Benefits of RBC Additive Solutions:
Produces an RBC concentrate of ___________ viscosity that is easier to infuse
Lower
Benefits of RBC Additive Solutions:
Allows harvesting of more plasma and platelets from the unit
T or F
T
What do you call the fluid inside the mother and daughter bags
Anticoagulant preservative
Additive solution is usually found in the ______ satellite bag
Last
Additive solution is added AFTER the removal of Plasma
T or F
T
Additive solutions:
Adsol (AS-_)
Nutricel (AS-__)
Optisol (AS-_)
SOLX (AS-_)
1,3,5,7
Additive solution allows to extend life of RBC or blood up to?
42 days
The additive solution must be added within ___ hours of WBC
72
When Adding Additive solution
_____mL for a 450mL blood
_____ mL for a 500mL blood
100
110
Chemicals in anticoagulant solution
Chelates Calcium
a. Citrate
b. Monobasic sodium phosphate
c. Dextrose
d. Adenine
e. Mannitol
a
Chemicals in anticoagulant solution
Maintains pH during Storage
a. Citrate
b. Monobasic sodium phosphate
c. Dextrose
d. Adenine
e. Mannitol
b
Chemicals in anticoagulant solution
Production of ATP
a. Citrate
b. Monobasic sodium phosphate
c. Dextrose
d. Adenine
e. Mannitol
d
Chemicals in anticoagulant solution
Substrate for ATP
a. Citrate
b. Monobasic sodium phosphate
c. Dextrose
d. Adenine
e. Mannitol
c
Chemicals in anticoagulant solution
Protect against storage-related hemolysis
a. Citrate
b. Monobasic sodium phosphate
c. Dextrose
d. Adenine
e. Mannitol
e
Process by which ATP and 2,3 DPG levels are restored or enhance by metabolic alterations
Rejuvenation solution
Process of reviving RBC
Rejuvenation solution
What are the Rejuvenation Solutio?
PIGPA
Pyruvate
Inosine
Glucose
Phosphate
Adenine
The only FDA approved rejuvenation solution
Rejuvesol
RBC storage Lesions:
Percent of viable cells
Glucose
ATP
pH
2,3 -DPG
Decrease or Increase
Decrease
RBC storage Lesions:
Plasma potassium
Plasma hemoglobin
Lactic acid
Decrease or Increase
Increase
in RBC storage lesions
Oxgen Dissociation curve is Shift to the right or left?
Shift to the Left
Pathogen Reduction technology includes
UV irradiation and Photo sensitizers
Psoralen Treatment
Decrease the the risk of transmissble blood disease
T or F
T
Removal of WBCs from blood or blood components prior to transfusion
Leukoreduction
Leukoreduction are given to patient with Anti-WBC
T or F
T
Febrile nonhemolytic transfusion reaction
Transfusion Related acute lung injury
Both of this requires leukoreduce
T or F
T
Residual WBC per each whole blood, RBC or apheresis Platelet
<5.0 x 10^6
<8.3 x 10^5
>85%
<5.0 x 10^6
_____ of the original component must be recovered after leukoreduction
<5.0 x 10^6
<8.3 x 10^5
>85%
85%
Residual WBC per each Platelets derived from whole blood
<5.0 x 10^6
<8.3 x 10^5
>85%
<8.3 x 10^5
Leukoreuduction categories:
Shortly after collection
a. Prestorage
b. Post-storage
a
Leukoreuduction categories:
Shortly after collection
a. Prestorage
b. Post-storage
a
Leukoreuduction categories:
At the patient’s bedside
a. Prestorage
b. Post-storage
b
Filters is made up of multiple layers of Polyester or cellulose acetate nonwoven fibers that trap leukocytes and platelets
T or F
T
Pore size: 17um
Removes fibrin clot
First generation Filters
second generation Filters
Third generation filters
First
Pore size: 20-40um
Removes micro aggregates
First generation Filters
second generation Filters
Third generation filters
Second
Removes 99.9% WBC
First generation Filters
second generation Filters
Third generation filters
Third
Pther methods to acheive RBC leukoreduction:
Centrifugation
Washing
Freezing
Thawing
Deglycerolizing
