9.2 Emulsion Test For Lipids

Question 1

How do you test for a lipid

Answer 1

1. Put a few drops of each sample into its own labelled test tube and add 2cm3 of ethanol shaking thoroughly
2. add 2cm3 of distilled water, shake gently
3. Record observations and conclusions: p/n, Holding the test tube on a white tile

Question 2

What are the steps to prepare a solid sample to test for lipid

Answer 2

Add the sample and 2cm3 of ethanol into a test tube. Use a glass rod to mash the solid, cleaning it between samples to avoid cross contamination. Shake it thoroughly and allow solid to settle at the bottom.

Question 3

Explain why you can’t see false positives for proteins or carbohydrates in lipids test

Answer 3

Proteins and carbohydrates do not dissolve in ethanol so cannot form a precipitate