4.3 effect of temp on enzyme

Question 1

What are the control variables for this enzyme practical

Answer 1

Temperature
substrate concentration and volume
volume of enzyme added

Question 2

How can we investigate the effect of temperature on the enzyme amylase

Answer 2

1. set up water baths 0,20,40,60,80
2. Put 1cm3 of both 1% starch and 1% amylase into 6 sperate test tubes and place in the water bath And leave to equilibrate
3. Use a pipette to place two drops of iodine solution into each well of a spotting tile
4. Add starch into amylase and start stopwatch
5. Every 10 seconds add a drop of the reaction solution into a well of iodine starting at zero
6. Note when no colour change occurs
7. Repeat for each temperature
8. Obtain results from two other groups with a same procedure to calculator mean
9. plot a rate graph

Question 3

Describe the effective temperature on enzyme graph

Answer 3

It will show an upside down U shape rate increases as temp increases but after optimum starts to fall again

Question 4

What are limitations for the rate of amylase investigation

Answer 4

The results are not quantitative as we didn’t use a colorimeter

Question 5

Why is it important to consider all variables in an investigation

Answer 5

As other variables such as enzyme concentration can change the rate of reaction in the results formed

Question 6

Why is PH not a control variable in the investigation of lipase activity

Answer 6

Lipase breaks down lipids into fatty acids which contain hydrogen So ph will vary