9: BASIC CONCEPTS ON LABORATORY BIOSAFETY AND BIOSECURITY Flashcards
- “The containment principles, technologies, and practices that are implemented to prevent unintentional exposure to pathogens and toxins, or their accidental release.”
- protects people from bad germs
BIOSAFETY
- “The protection, control, and accountability for valuable biological materials within laboratories, in order to prevent their unauthorized access, loss, theft, misuse, diversion or intentional release”
- protects germs from bad people
BIOSECURITY
- is terrorism involving the intentional release or dissemination of biological agents.
BIOTERRORISM
environmental health engineer for Dow Chemical Company; 1966
- Charles Baldwin
- Used in labeling biological materials carrying significant health risks
o Striking – to draw immediate attention
o Unique and unambiguous
o Quickly recognizable and easily recalled
o Easily stenciled
o Symmetrical
o Acceptable to groups of varying ethnic backgrounds
BIOSAFETY SYMBOL
The host from which the microorganism originates. The carrier host might not show symptoms.
- Source
The organism in which the microorganism infect. The new host must be susceptible.
- Host
The type of microorganism that causes infection or hazardous condition.
- Agent
The means of transmission, mostly direct or indirect. Some routes of transmission include air, insect, direct contact and contaminated surfaces.
Transmission
- began in 1943
- US Biological Weapons Program
- ordered by President Franklin Roosevelt
Biosafety
first scientific director
- Ira L. Baldwin
designed modifications in the Camp
- Newell A. Johnson
- 1907-1908
- Described the use of mechanical pipettors to prevent laboratory acquired infections
- Recognized as the Pioneer of Biosafety
ARNOLD WEDUM
- A pharmaceutical company in Pennsylvania developed ventilated cabinet to prevent infection from M. tuberculosis
1909
- smallpox outbreak
- WHO aggressively pursued eradication of smallpox virus
- WHO Assembly consolidated the remaining virus stocks to:
o Center for Disease Control (CDC) - U.S.A.
o State Research Center of Virology and Biotechnology VECTOR (SRCVB VECTOR) – Russia
1967
- CDC Published the Classification of Etiological Agents on the Basis of Hazard
1974
- National Institutes of Health (NIH) published Guidelines for Research Involving Recombinant DNA Molecules
1976
- WHO released the first edition of Laboratory Biosafety Manual
1983
- CDC and NIH released the first edition of Biosafety in Microbiological and Biomedical Laboratories
- American Biological Safety Association (ABSA)
1984
- US government enacted the Select Agent Regulations
- Monitor transfer of select list of biological agents from one facility to another.
1996
- Amerithrax
- Revised Select Agent Regulations
- required specific security measures for any facility that used or stored one or more agents on the new, longer list of agents.
2001
- Revision of Select Agent Regulations
- Creation of two tiers of select agents
- Tier 1 agents pose greater risk of deliberate misuse.
2012
Provides international regulatory
framework to ensure “an adequate level
of protection in the field of safe transfer, handling, and use of living modified organisms (LMOs) resulting from modern biotechnology”.
- Cartagena Protocol on Biosafety (2003) – UN
- Laboratory Biosafety Manual, 1983
- Containment levels 1-4.
- Laboratory Biosafety Manual – WHO