9

Question 1

2 types of sterility tests

Answer 1

membrane filtration test and direct inoculation

Question 2

membrane filtration test 3 steps

Answer 2

1. filtration though 0.45 micrometer filter
2. culture
3. incubate

Question 3

what does filtration do in membrane filtration sterility testing

Answer 3

it removes preservatives or ABs that may inhibit growth or cause turbidity

Question 4

3 steps in direct inoculation sterility testing

Answer 4

1. aseptic removal of sample
2. inoculation in culture medium
3. incubation

Question 5

3 issues w direct inoculation method

Answer 5

1. small volume of product causes low sensitivity
2. may need neutralisation of product has antomicorbial properties
3. cloudy sample can affect reading

Question 6

dry heat sterilisation os good for

Answer 6

materials that may be damaged by moist heat or are impenetrable tp moms heat eg powders, petroleum products and sharp instruments

Question 7

which is of the 2 types of dry heat sterilisation methods is better

Answer 7

forced air is better than static air

Question 8

temperature used for dry heat sterilisation

Answer 8

220 degree celsius

Question 9

which method of sterilisation can remove endotoxins

Answer 9

dry heat sterilisation

Question 10

what are examples of chemical sterilants

Answer 10

ethylene oxide, peracetic acid, h2o2 fog, formaldehyde

Question 11

issues w ethylene oxide

Answer 11

possible carcinogen
eye pain sotre throat, convulsions
flammable and explosive

Question 12

ethylene oxide residue

Answer 12

removed via aeration

Question 13

peracetic acid efficacy in organic soil

Answer 13

remains but reduced

Question 14

activity of peracetic acid

Answer 14

fungi, bacteria and yeast
not viruses

Question 15

peracetic acid must be combined with

Answer 15

anticorrosive agent eg sulfonate
temp round 50C

Question 16

H2o2 plasma temperature

Answer 16

37-44 (lowest of all chemical sterilants)

Question 17

range of efficacy of h2o2 plasmas

Answer 17

wide range including, Cdif, mycobacteria, MRSA

Question 18

byproduct toxicity of h2o2 plasma

Answer 18

non toxic byproducts water and oxygen

Question 19

issue w h2o2 plasma

Answer 19

low penetration so yo need a penetration enhancer

Question 20

Gamma radiation uses

Answer 20

cobalt 60
cesium 137
electron accelerators

Question 21

temp of gamma radiation

Answer 21

not raised at all

Question 22

Mao of gamma radiation

Answer 22

disrupts nucleic acids

Question 23

gamma radiation is not suitable for

can also cause

Answer 23

aqueous products with proteinaceous component
possible delimitation, oxidation and cracking in polyethylene

Question 24

ozone as a sterilant effectiveness

Answer 24

affects spores and all types of microbes

Question 25

stability of ozone

Answer 25

not stable but is good as the residue can be removed by leaving product in chamber for a few minutes

Question 26

advantage of ozone

Answer 26

compatibility with many materials

Question 27

nosocomial infections show resistance to

Answer 27

AGs, quinolone, cephalosporins, extended spectrum penicillins

Question 28

risk classification of patients

Answer 28

A B C
A has minimal risk (no underlying disease)
B has infection
C is severely immunocompromised or has severe burns or trauma

Question 29

low level disinfectants - 4

Answer 29

alcohol, sodium hypochlorite (bleach), iodophors, phenols

Question 30

high level Ds - 6
can affect spores

Answer 30

gluteraldehyde, h2o2 at 7.5%, ortho pthaldehyde, h2o2/peracetic acid, formaldehyde, sodium hypochlorite

Question 31

is QAC high or low level D

Answer 31

low

Question 32

are high level disinfectants sporicidal

Answer 32

no but at prolonged exposure time they can be and will be chemical sterilants