7

Question 1

mic

Answer 1

lowest conc that inhibits growth (bacteriostatic)

Question 2

mec

Answer 2

lowest conc of active ingredient at which product is still effective (bactericidal)

Question 3

min recommended conc

Answer 3

conc that should be used for common practice

Question 4

mbc

Answer 4

min bactericidal con that causes at least a 3 log reduction in no of surviving cells after incubation.

Question 5

min lethal conc

Answer 5

same as Mac but test can be directed towards any microbe

Question 6

MSC

Answer 6

min selective conc at which resistant strains have a comp advantage. at MSC benefit of bacterial growth exceeds fitness cost of carrying the drug resistant trait compared with resistant strain

Question 7

7 steps for evaluation test

Answer 7

1. prep carrier
2. prep test organism and inoculum
3. inoculation, drying and transfer of carriers
4. exposure of dried inoculum to test substance or control fluid
5. neutralisation of test substance and elution of test organism
6. dilution and recovery of test organism
7. counting the surviving test organisms and assessing performance of test substance

Question 8

types of tests

Answer 8

suspension test, carrier test, in use and field test

Question 9

in use and field test

Answer 9

can be carrier or suspension test. not a quantitative approach to reduction of flora (no residual contamination)

Question 10

diluent for carrier test

Answer 10

standardised hard water

Question 11

is hand rub or hand wash more efficacious

Answer 11

hand rub

Question 12

eucalyptus essential oil more effective against

Answer 12

e coli than stash aureus

Question 13

is neutralisation performed before or after contact time with test product and why

Answer 13

after, to stop lingering effect of disinf

Question 14

what is the virucidal efficacy success criteria

Answer 14

at least a 4 log reduction in kjmcxz