8.9 Flashcards
All of the following are examples of appropriate specimens for the recovery of fungi except:
A. Tissue biopsy
B. CSF
C. Aspirate of exudate
D. Swab
D. Swab
For which clinical specimens is the KOH direct mount technique for examination of fungal elements used?
A. Skin
B. CSF
C. Blood
D. Bone marrow
A. Skin
The India ink stain is used as a presumptive test for the presence of which organism?
A. Aspergillus niger in blood
B. Cryptococcus neoformans in CSF
C. Histoplasma capsulatum in CSF
D. Candida albicans in blood or body fluids
B. Cryptococcus neoformans in CSF
Cutaneous disease involving skin, hair, and nails usually indicates an infection with a:
A. Dimorphic fungus
B. Dermatophyte
C. Zygomycetes
D. Candida species
B. Dermatophyte
What is the first step to be performed in the identification of an unknown yeast isolate?
A. Gram stain smear
B. India ink stain
C. Catalase test
D. Germ tube test
D. Germ tube test
An isolate produced a constriction that was interpreted as a positive germ tube, but Candida albicans was ruled out when confirmatory tests were performed. Which of the following fungi is the most likely identification?
A. Candida tropicalis
B. Cryptococcus neoformans
C. Candida glabrata
D. Rhodotorula rubra
A. Candida tropicalis
Cornmeal agar wit Tween 80 is used to identify which characteristic of an unknown yeast isolate?
A. Hyphae (true and pseudo)
B. Blastoconidia and arthroconidia
C. Chlamydospores
D. All of these options
D. All of these options
Blastoconidia are the beginning of which structures?
A. Arthroconidia
B. Germ tubes
C. Pseudohyphae
D. True hyphae
C. Pseudohyphae
An isolate from CSF growing on cornmeal agar produces the following structures:
-Blastoconidia=+
-Chlamydospores=Neg
-Pseudohyphae=Neg
-Arthroconidia=Neg
Which test should be performed next?
A. Birdseed agar and urease
B. Germ tube and glucose
C. India ink and germ tube
D. All of these options
A. Birdseed agar and urease
Which of the following yeast enzymes is detected using birdseed (niger seed) agar?
A. Phenol oxidase
B. Catalase
C. Urease
D. Nitrate reductase
A. Phenol oxidase
C. neoformans; R. rubra; T. cutaneum; C. albicans
Urease: +, +, +, Neg
Germ tube: Neg, Neg, Neg, +
Brown and black colonies on niger seed agar: +, Neg, Neg, Neg
Which of the following yeast is characteristically positive for germ tube production?
A. Candida tropicalis
B. Candida kefyr (pseudotropicalis)
C. Cryptococcus neoformans
D. Candida albicans
D. Candida albicans
Arthroconidia production is used to differentiate which two yeast isolates?
A. Candida albicans and Candida dubliniensis
B. Trichosporon cutaneum and Crytococcus neoformans
C. Candida albicans and Candida tropicalis
D. Saccharomyces cerevisiae and Candida glabrata
B. Trichosporon cutaneum and Crytococcus neoformans
The urease test, niger seed agar test, and the germ tube test are all used for the presumptive identification of:
A. Rhodotorula rubra
B. Cryptococcus neoformans
C. Trichosporon cutaneum
D. Candida albicans
B. Cryptococcus neoformans
Blastoconidia; Pseudohyphae; Arthroconidia
Crytococcus spp: +, Neg, Neg
Candida spp.: +, +, Neg
Trichosporon spp.: +, +, +
Geotrichum: Neg, +, +
Which of the following yeasts produces only blastoconidia on cornmeal Tween 80 agar?
A. Candida spp.
B. Trichosporon spp.
C. Geotrichum spp.
D. Crytococcus spp.
D. Crytococcus spp.
Ascospores are formed by which yeast isolate?
A. Saccharomyces cerevisiae
B. Candida albicans
C. Cryptococcus neoformans
D. All of these options
A. Saccharomyces cerevisiae
A germ tube-negative, pink yeast isolate was recovered form the respiratory secretions and urine of a patient with AIDS. Given the following results, what is the most likely identification?
Cornmeal Tween 80 Agar
-Blastoconidia=+
-Arthroconidia=Neg
-Pseudohyphae=Neg
-Urease=+
A. Candida albicans
B. Rhodotorula spp.
C. Crystococcus spp.
D. Trichosporon spp.
B. Rhodotorula spp.
Chlamydospore production is demonstrated by which Candida species?
A. C. glabrata
B. C. krusei
C. C. albicans
D. C. tropicalis
C. C. albicans
Carbohydrate assimilation tests are used for the identification of yeast isolates by inoculating media:
A. Free of carbohydrates
B. Free of niger seed
C. Containing carbohydrates
D. Containing yeast extract
A. Free of carbohydrates
Yeast recovered form the urine of a catheterized patient receiving chemotherapy for cancer gave the fllowing results:
Cornmeal Tween 80 agar
-Germ tube=+
-Pseudohyphae=+
-Blastoconidia=+
-Arthroconidia=Neg
-Chlamydospores=+
What further testing is necessary?
A. Carbohydrate assimilation and urease
B. Urease and niger seed
C. Nitrate reductase and carbohydrate fermentation
D. No further testing is needed for identification
D. No further testing is needed for identification
A blood agar plate inoculated with sputum from a patient with diabetes mellitus grew very few bacterial flora and predominance of yeast. Given the following results, what is the most likely identification of the yeast isolate?
Cornmeal Tween 80 Agar
-Germ tube=Neg
-Arthroconidia=Neg
-Chlamydospores=Neg
-Pseudohyphae=+
-Blastoconidia=+ (arranged along pseudohyphae)
A. Candida tropicalis
B. Candida kefyr (pseudotropicalis)
C. Trichosporon cutaneum
D. Geotrichum candidum
A. Candida tropicalis