8.6 Flashcards

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1
Q

Large gram-positive spore-forming rods growing on blood agar as large, raised, B-hemolytic colonies that spread and appear as frosted green-gray glass are most likely
A. Pseudomonas spp.
B. Bacillus spp.
C. Corynebacterium spp.
D. Listeria spp.

A

B. Bacillus spp.

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2
Q

Bacillus anthracis and Bacillus cereus can best be differentiate by which test?
B-hemo; motility; oxidase; catalase; lecithinase; glucose
B.cereus: +; +; Neg: +; +; +
B.anthracis: Neg; Neg; Neg: +; +; +
A. Motility and B-hemolysis on a blood agar plate
B. Oxidase and B-hemolysis on a blood agar plate
C. Lecithinase and glucose
D. Lecithinase and catalase

A

A. Motility and B-hemolysis on a blood agar plate

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3
Q

Which is the specimen of choice for proof of food poisoning by Bacillus cereus?
A. Sputum
B. Blood
C. Stool
D. Food

A

D. Food

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4
Q

A suspected Bacillus antrhacis culture obtained from a wound specimen produced colonies that had many outgrowths (Medusa-head appearance), but were not B-hemolytic on sheep blood agar. Which test should be performed next?
A. Penicillin (10-unit) susceptibility test
B. Lecithinase test
C. Glucose test
D. Motility test

A

A. Penicillin (10-unit) susceptibility test

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5
Q

Which of the following test should be performed for initial differentiation of Listeria monocytogenes from group B streptococci?
A. Gram stain, motility at room temperature, catalase
B. Gram stain, Camp test, H2S/TSI
C. Oxidase, CAMP test, glucose
D. Oxidase, bacitracin

A

A. Gram stain, motility at room temperature, catalase

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6
Q

Culture of a finger wound specimen from a meat packer produced short gram-positive bacilli on a blood agar plate with no hemolysis. Given the following test results at 48 hours, what is the most likely identification?
-Catalase=Neg
-Motility(wet prep)=Neg
-Motility (media)=Neg (bottle-brush growth in stab culture)
A. Bacillus cereus
B. Listeria monocytogenes
C. Erysipelothrix rhusiopathiae
D. Bacillus subtilis

A

C. Erysipelothrix rhusiopathiae

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7
Q

A non-spore-forming, slender gram-positive rod forming palisades and chains was recovered from a vaginal culture and grew well on tomato juice agar. The most likely identification is:
A. Lactobacillus spp.
B. Bacillus spp.
C. Neisseria spp.
D. Streptococcus spp.

A

A. Lactobacillus spp.

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8
Q

A Corynebacterium species recovered from a throat culture is considered a pathogen when it produces:
A. A pseudomembrane of the oropharynx
B. An exotoxin
C. Gray-black colonies with a brown halo on Tinsdale’s agar
D. All of these options

A

D. All of these options

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9
Q

A presumptive diagnosis of Gardnerella vaginalis can be made using which of the following findings?
A. Oxidase and catalase tests
B. Pleumorphic bacilli heavily colonized on vaginal epithelium
C. Hippurate hydrolysis test
D. All of these options

A

D. All of these options

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10
Q

A gram-positive branching filamentous organism recovered from a sputum specimen was found to be positive with a modified acid-fast stain method. What is the most likely presumptive identification?
A. Bacillus spp.
B. Nocardia spp.
C. Corynebacterium spp.
D. Listeria spp.

A

B. Nocardia spp.

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11
Q

Routine laboratory testing for Treponema pallidum involves:
A. Culturing
B. Serological analysis
C. Acid-fast staining
D. Gram staining

A

B. Serological analysis

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12
Q

Spirochetes often detected in the hematology laboratory, even before the physician suspects the infections, are:
A. Borrelia spp.
B. Treponema spp.
C. Campylobacter spp.
D. Leptospira spp.

A

A. Borrelia spp.

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13
Q

Which of the following organisms is the cause of Lyme disease?
A. Treponema pallidum
B. Neisseria meningitidis
C. Babesia microti
D. Borrelia burgdorferi

A

D. Borrelia burgdorferi

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14
Q

The diagnostic method most commonly used for the identification of Lyme disease is:
A. Serology
B. Culture
C. Gram stain
D. Acid-fast stain

A

A. Serology

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15
Q

Primary atypical pneumonia is caused by:
A. Streptococcus pneumoniae
B. Mycoplasma pneumoniae
C. Klebsiella pneumoniae
D. Mycobacterium tuberculosis

A

B. Mycoplasma pneumoniae

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16
Q

Which organism typically produces “fried-egg” colonies on agar within 1-5 days of culture from a genital specimen?
A. Mycoplasma hominis
B. Borrelia burgdorferi
C. Leptospira interrogans
D. Treponema pallidum

A

A. Mycoplasma hominis

17
Q

The manganous chloride-urea test is used for the identification of which organism?
A. Mycoplasma pneumoniae
B. Ureaplasma urealyticum
C. Bacillus cereus
D. Borrelia burgdorferi

A

B. Ureaplasma urealyticum

18
Q

A gram-positive (gram-variable), beaded organism with delicate branching was recovered from the sputum of a 20-year-old patient with leukemia. The specimen produced orange, glabrous, waxy colonies on Middlebrook’s agar that shoed partial acid-fast staining with the modified Kinyoun stain. What is the most likely identification?
A. Rhodococcus spp.
B. Actinomadura spp.
C. Streptomyces spp.
D. Nocardia spp.

A

D. Nocardia spp.

19
Q

A direct smear form a nasopharyngeal swab stained with Loeffler methylene blue stain showed various letter shapes and deep blue, metachromatic granules. The most likely identification is
A. Corynebacterium spp.
B. Nocardia spp.
C. Listeria spp.
D. Gardnerella spp.

A

A. Corynebacterium spp.

20
Q

Which of the following is the best, rapid, noncultural test to perform when Gardnerella vaginalis is suspected in a patient with vaginosis?
A. 10% KOH test
B. 3% H2O2 test
C. 30% H2O2 test
D. All of these options

A

A. 10% KOH test

21
Q

Which is the test of choice for the confirmation of Chlamydia trachomatis infection in urine?
A. Enzyme immunoassay antigen testing
B. PCR molecular testing
C. Culture using McCoy and Hela cells
D. Microimmunofluorescence (MIF) test

A

B. PCR molecular testing

22
Q

Which test is the most reliable for the detection of Mycoplasma pneumonia in serum and for the confirmation of diagnosis?
A. EIA testing and direct antigen testing
B. Cold agglutinin testing using Group O RBCs
C. Culture on SP4 glucose broth with arginine
D. Complement fixation

A

A. EIA testing and direct antigen testing

23
Q

Identify the following bacterium and specimen pairing that is mismatched (specimen not appropriate for isolation).
A. Chlamydia (Chlamydophila) psittaci: fecal swab
B. Chlamydia trachomatis: first voided urine
C. Chlamydia trachomatis: endocervical swab
D. Chlamydia pneumonia: throat swab or sputum

A

A. Chlamydia (Chlamydophila) psittaci: fecal swab

24
Q

Which of the following organisms are transmitted to animals and humans after a tick bite?
A. Leptospira
B. Chlamydia and Mycoplasma spp.
C. Neisseria sicca
D. Ehrlichia and Anaplasma spp.

A

D. Ehrlichia and Anaplasma spp.

25
Q

Following a hike in the woods, a young male noted a tick on his ankle. He removed the tick, but 2 weeks later noticed a circular, bull’s eye rash at the site of the bit. Which specimen(s) should be obtained to establish a diagnosis of Lyme borreliosis?
A. Lymph node biopsy, skin scraping
B. Blood, CSF, and skin biopsy
C. Hair, fingernails
D. Saliva, sputum

A

B. Blood, CSF, and skin biopsy