8.4 Transcription And Splicing Flashcards
Transcription definition
Process of make pre- mRNA using part of the DNA as a template
Splicing definition
Process of making mRNA from pre-mRNA by removing its introns
Transcription process
- chains part
- breaking of hydrogen bonds
- by DNA Helicase
- RNA bases line up with complementary bases on template strand
- phosphodiester bonds form between mononucleotides by condensation reactions
- by RNA polymerase
- pre- mRNA spliced to remove introns
Where does transcription and splicing occur?
Nucleus
How does RNA polymerase know when to stop?
When the RNA polymerase reaches a the ‘stop’ triplet code
What is the product of transcription in prokaryotic cells?
DNA -> mRNA
What is the product formed by transcription in eukaryotic cells?
DNA -> pre- mRNA
Why is splicing unnecessary in prokaryotes?
No introns
Why are introns removed?
Prevent the synthesis of a polypeptide as they don’t code for anything
How does mRNA leave the nucleus?
Through nuclear pores
Why don’t mRNA just diffuse out of cells?
Too large
what is the product of transcription in prokaryotes?
mRNA
what is the product of transcription in eukaryotes?
pre- mRNA
what do eukaryotes have that prokaryotes don’t?
introns