8. Isothermal NAAT

Question 1

What is HPA

Answer 1

Hybridization protection assay

Question 2

TMA is an RNA amplification method driven by what 2 enzymes?

Answer 2

Reverse transcriptase

RNA polymerase

Question 3

Reagent added to a completed TMA reaction to perform a Hybridization Protection Assay (HPA)

Answer 3

Acridinium ester (AE)-labeled DNA probes

Question 4

How does isothermal amplification differ from PCR?

Answer 4

Target amplification occurs at a SINGLE temperature

Question 5

Relative to PCR, how does TMA reduce the risk of false positives in subsequent assays?

Answer 5

TMA produces RNA amplicons

They’re unstable outside reaction tube = low risk as a contaminant