3. Enzymes for NAT

Question 1

What is RT-PCR

Answer 1

Reverse transcriptase PCR

Where RNA first converted to DNA, then the DNA is amplified

Question 2

Enzyme used in transcription-mediated amplification (TMA) and RT-PCR

Answer 2

Reverse transcriptase (RT)

Question 3

4 components of a simple restriction digest in order of combining

Answer 3

1. Nuclease-free water
2. RE digest buffer
3. dsDNA to be cut
4. Restriction enzyme

Question 4

RNA polymerase template requirements

Answer 4

dsDNA promoter sequence from which to initiate ssRNA synthesis

Question 5

RNA polymerase is ___-directed and has ___ polymerase activity

Answer 5

DNA-directed

RNA polymerase activity

Question 6

What is star activity

Answer 6

RE cuts at sequences similar to, but slightly different than, specific restriction sites

Question 7

Taq has __’ to __’ polymerase function and __’ to __’ exonuclease function

Answer 7

5’-3’ for both polymerase and exonuclease function

Question 8

Reverse transcriptase is ___-directed and has ___ polymerase activity

Answer 8

RNA-directed

DNA polymerase activity