1. Nucleic Acid Structure and Chemistry

Question 1

Difference between nucleoside and nucleotide

Answer 1

Nucleoside = sugar + base

Nucleotide = sugar + base + 5’ phosphate(s)

Question 2

Word for ‘between different nucleic acid strains’

Answer 2

INTER-molecular

Question 3

Bond that links adjacent nucleotides together at 5’ C

Answer 3

Phosphodiester

Question 4

Bond that links the deoxyribose moiety to the base at C1’

Answer 4

Glycosidic

Question 5

What type of bond holds 2 complementary strands of DNA together?

Answer 5

Hydrogen

Question 6

Loss of secondary structure (reversible)

Answer 6

Denaturation

Question 7

Acid conditions promote hydrolysis of these 2 bonds resulting in loss of primary DNA structure

Answer 7

• Glycosidic

- Phosphodiester

Question 8

What occurs at C1’ of a deoxynucleotide

Answer 8

Glycosidic linkage with base

Question 9

Linkage found between 5’ and 3’ carbons of adjacent nucleotides of the same strand

Answer 9

Phosphodiester

Question 10

Loss of primary structure

Answer 10

Degradation

Question 11

Word for ‘within the same nucleic acid strand’

Answer 11

INTRA-molecular

Question 12

How do DNA nucleases destroy primary DNA structure

Answer 12

By hydrolyzing phosphodiester linakges between adjacent deoxyribose sugars

Question 13

Difference between C2’ position of DNA vs RNA

Answer 13

DNA - H

RNA - OH