7.1 Using gene sequencing

Question 1

What is a definition of a genome

Answer 1

• All of an organisms DNA

Question 2

What is the aim of PCR

Answer 2

• To amplify a sample of DNA

Question 3

Explain the stages in PCR

Answer 3

1 - A culture is set up including : DNA primers, Free nucleotides, primers and heat resistant DNA polymerase
2 - Mixture is then heated up to break the hydrogen bonds between DNA and form two strands
3 - Mixture is then cooled so the primers can then bind to the strand