7 - Measuring Protein Activity Flashcards
1
Q
what can be found from measuring proteins?
A
conc
function
activity
2
Q
why is spectroscopy the desired method?
A
macromolecules can absorb electromagnetic radiation
3
Q
types of spec?
A
- NMR
- infared
- ultra v
4
Q
examples of molceules that absorb at specific wavelengths?
A
dna - 260
aromatic residues - 280
peptide bonds - 200-300
5
Q
how do molecules have different absorptions?
A
as they only absorb a wavelength that will change its state from ground - excited
6
Q
express absorbtion as an eq?
A
.
