6 - Protein Interactions Flashcards
1
Q
What can proteins interact with?
A
Ligand Proteins Membranes Dna/rna Sugars
2
Q
Why are proteins described as complexes?
A
Have domains that work together
3
Q
Types of bond when a protein interacts with a lipid?
A
Non-covalent
4
Q
Types of bond when a protein interacts with another protein?
A
Covalent
Between the domains of the proteins
5
Q
What can increase likelihood of proteins interacting with each other?
A
Availability
Competition
Matching non-cov bonds [like ionic]
6
Q
Factors that can affect protein inter?
A
Ph
Cellular localisation
Ligand
7
Q
How are protein-ligand interactions measured?
A
Graph showing bound protein abasing conc of ligand
Affinity indicated by Kd
- conc of substrate/ligand needed to saturate 50% of protein
