10 - Energetics and Dynamics

Question 1

Lineweaver Burk equation from MM?

Answer 1

mm
Vo = Vmax [S] / Km + [S]

LB
1/Vo = Km/Vmax[S] + 1/Vmax

Question 2

how can LWB be used to plot a line?

Answer 2

eq of line y = mx + c

so

y= 1/Vo

M= Km/Vmax

x = 1/[S]

c = 1/Vmax

Question 3

how to get y and x intercept?

Answer 3

y is in eq and is the 1/Vmax part

x
-1/Km

Question 4

effect on Km and Vmax in COMPETITIVE inhibition?

Answer 4

Km increases [affinity for en]

Vmax not changed [Substrate outcompetes inhibitor]

Question 5

effect on Km and Vmax in NON-COMPETITIVE inhibition?

Answer 5

Km not changed

Vmax reduced [the release of prods bclyl and Non comp inhib stops this so it decreases]

Question 6

natural and synthetic competitive inhibitor?

Answer 6

nat: tetradotoxin [Na channels]
syn: Ibuprofen [COX inihib]

Question 7

natural and synthetic non-competitive inhibitor?

Answer 7

nat: Caffiene [camp phosphodiesterase]
syn: Haloperidol [NO synthase]

Question 8

plotting LB graph?

Answer 8

x