7. Blood Donor Testing Flashcards
PLTs are stored at ___ for up to __ days
RT; 7 days
HTLV confirmatory test?
HTLV Western blot
a common community acquired infection that lies dormant in white blood cells and is harmless for most of the population
Cytomegalovirus (CMV)
Phenotyping for these for 6 are routinely performed on a subset of repeat donor sample
C/c E/e Kell Duffy Kidd S/s
3 things that happen during a lookback investigation?
- Donor deferred; their previous donations are investigated
- Hospitals that received blood are notified and asked to ID recipients
- Recipients are tested
Environment where syphilis can’t survive
72 hrs in citrated blood at 1-6 degC
72 hrs at RT
The process of identifying and contacting recipients of blood components from a donor who, on a subsequent donation or testing, is confirmed to have tested positive for the presence of an infectious agent
LOOK BACK
This is the process of investigating a report of transfusion-associated infection in blood recipients who have already received blood components.
TRACEBACK
The only component capable of transmitting syphilis infection
Platelets
Purpose of TRACEBACK investigation?
Determine if any donor who provided blood for that recipient has tested positive for an infectious agent
_____ has been associated with adult T-cell leukemia
and myelopathy / tropical spastic paraparesis (HAM/TSP)
HTLV-I
Why is the risk of CMV infection related to blood products low in Canada
CMV = cell-associated virus and cellular components get LEUKOREDUCED in Canada
How often can plateletpheresis be done?
Every 2 weeks (14 days)
Minimum weight requirement to donate?
50kg/110lbs
Disease caused by Treponema pallidum
Syphilis
How does MHA-TP work
Sheep RBC coated w/ T. pallidum antigen => test for agglutination w/ patient plasma
How often can plasmapheresis be done?
Every week (7 days)
When would chagas be tested?
Donor is “at-risk” as indicated by questionnaire
Why are platelets the only component capable of transmitting syphilis?
Storage of PLTs is at RT (cannot survive cold)
Minimum Hgb requirements for male and female
Female 125 g/L
Male 130 g/L
Assay used to detect antibody to T. cruzi
Chemiluminescent
________ transfusions require CMV negative products
Intrauterine
How often can male donors donate whole blood? Female?
Male - 56 days
Females - 84 days
Test for Treponema pallidum
MHA-TP micro hemagglutination test
