6d Culturing Microorganisms (triple only) Flashcards
Name the process used by bacteria to reproduce
Binary fission
How frequently can bacteria reproduce given ideal contidionts?
Every 20 mintues
Name the two mediums that can be used in the lab to culture bacteria
Nutrient broth
Agar gel plate
Describe how an uncontaminated cuture of bacteria can be predpared using aseptic techniques
Sterilise petri dish
Sterilise culture media (agar gel or nurtient broth)
Sterilise inoculating loop by passing through a flame
Use inoculatinf loop to trasnfer micororganisms to the sterile medium
Secure the lid of Petri dish with adhesive tape
Store plate upside down
Incubate at 25oC
Why is the petir dish sealed with two pieces of adhesive tape?
Secures lid
Allows oxygen to diffuse inot the Petri dish
State the nutrients foudn in culture medium
Water
Glucose
Amino acids
Minerals
The picture shows an incubated pertir dish containing bacteria,.
What is the name given to the ‘dots’ of bacteria?
Colony
Why are school unable to incuabate bactrerial cultures at 37oC
Human pathogens would grow and increase in numbers
What is the name of the clear area around the filter paper discs on the plate?
Zone of inhibition
How could the area of the zone of inhibition labelled A be calculated?
Measure the diameter of the inhibition zone
Divide by 2 to get the radius
Use the equation
πr2
