6.6 michealis-menten

Question 1

catalytic groups are

Answer 1

residues also known as the active site, usually hydrophilic. participate in making and breaking of bonds. uniquer microenvironments

Question 2

mechanism for non allosteric enzyme catalyzed rxn

Answer 2

E + S <—> ES -> E+P

ES complex can dissocitae to E and S with a rate of k_-1 or proceed to porduct P with rate constant of k₁

Question 3

which part is fast and reversible

Answer 3

E+S <–> ES forward rxn rate constant is k₁ reverse is k_-1

Question 4

at low levels of substrate what order is the rxn

Answer 4

enzyme concentration is consant but substrate varies. low levels of substrate means first order, the velocity depends on [S]

Question 5

at highler levels of substrate what is the order

Answer 5

zero order, active sites of all enzymes are saturated

Question 6

given M-M graph what is v_max

Answer 6

At an infinite subste [], reaction would proceed to its maximum velocity

Question 7

what is k_m

Answer 7

k_m is the substrate concetration when the rxn proceeds at one half its max velocity

Question 8

what does the lower the k_mmean

Answer 8

te higher the affinity of the enzyme for the substrate