4.5 Conformational changes accompanying hemoglobin fn

Question 1

which other ligands are involved in cooperative effects when O2 binds to hemoglobin

Answer 1

H+ and CO2 which bind to hemoglobin affect affinity of O2 by altering 3-D strurcture

Question 2

the effect of H+ on hemglobin is called

Answer 2

the Bohr effect

Question 3

is the oxygen binding ability of myoglobin afftected by H+ or CO2

Answer 3

no

Question 4

whats happens to affinity w/increase in H+

Answer 4

lowers pH, reduces O2 affinity of hemoglobin, increasing H+ causes protonation of key aa including N-terminals of alpha chains and His146 of beta chains. protnated his stablized by salt bridge of Asp94, this favors deoxygenated form

Question 5

one way environment is acidic

Answer 5

actively metabolzing tissure require oxygen, releases H+