6.-Enzymes-in-Action-Planning-the-Practical Flashcards
What are the four structures of proteins?
Primary, secondary, tertiary, and quaternary structure.
What is denaturation?
A major, irreversible change in proteins caused by structural changes due to pH or temperature.
How is the structure of an enzyme formed?
By various types of interactions between different amino acid R groups.
What does the lock and key model describe?
The specificity of a substrate molecule fitting into the active site of a specific enzyme.
What happens to enzymes when exposed to extremes of pH or high temperatures?
The shape of their active site may change, leading to denaturation.
What is egg albumen?
The mixture of proteins found in egg whites, primarily consisting of ovalbumin and lysozyme.
What should be considered when planning a practical investigation?
Choice of equipment, hazards and risks, methods, variables, and how to record results and observations.
What are the types of variables in an investigation?
Independent variables, dependent variables, and control variables.
What equipment is provided for the investigation?
Egg albumen, solutions of different pH, thermometers, test tubes, water-baths at different temperatures, and a stopwatch.
What should be included in the methods for each investigation?
Setup of equipment, suitable values for independent variables, number of trials, control of unwanted variables, and assessment of effects.
What is required in a risk assessment?
Identification of potential hazards, who could be harmed, risk probability, minimization strategies, and incident procedures.
What should be done with results and observations?
Identify how to record them and consider how to present the data.
What are the learning objectives for Lessons 3 and 4?
To carry out investigations into the effects of pH and temperature on egg albumen, work safely, record results in tables, and write valid conclusions.
