5/19

Question 1

tRNA

Answer 1

• Binds to amino acid at 3’ end and has CCA at each 3’ end that binds AA
• Reads complementary to mRNA

Question 2

Amino-acyl tRNA synthestase

Answer 2

• Is necessary for the correct AA pairing with the right tRNA
• Uses ATP to form bond between tRNA and AA, this energy is what is used to catalyze peptide bond formation
• Aminoacyl tRNA is charged and can bind A site (inhibited by tetracyclines)

Question 3

Initition

Answer 3

Requires intiating factors to bind ribosome and hold structure
-Once mRNA is bound then initation factors fall off

Question 4

Elongation

Answer 4

• aminoacyl tRNA binds to A site on 30s, bond is catalyzed in P site and moves off in E site
• GTP is used for the translocation step

Question 5

Termination

Answer 5

Poly A tail is necessary for termination,

Stop codons UAA, UGA,UAG

Question 6

Antibiotics

Answer 6

• Aminoglycosides bind 30S and cause misreading at initiation
• Macrolides bind 50S and prevent leaving of tRNA
• Chloramphenicol blocks transpeptidation

Question 7

RER

Answer 7

• N linked sugar added
• Also site of collagen hydroxylation
• Enriched in mucus cells and plasma cells

Question 8

Golgi

Answer 8

-N linked sugars and modification of O linked
-Attachement of M6P and other signals
-Clathrin is from cell surface, COPI is in and COPII is out
-

Question 9

I cell diesease

Answer 9

• Golgi can’t add M6P and so can’t send lyosomal enzymes to lysosome
• Increased enzymes in blood, cluding of corneas, joint immobility,

Question 10

Angelmanns Symdrome

Answer 10

• inaproporiate laaughter, imprinting of fathers leading to only expression of mothers
• Happy demeanor, siezures, retardation
• Protein is an E3 ligase

Question 11

VHL

Answer 11

• Vascular tumors and siezures

- mutation is E3 ligase

Question 12

Microtubule Drugs

Answer 12

Vincristine, Blastine, mebednazole, griseofulvin all destabalize
-Paclitaxel stabalizes in locked position

Question 13

Vimentin

Answer 13

Connective Tissue

Question 14

Keratin

Answer 14

Epithelium

Question 15

Karterengers

Answer 15

• Ciliary dyskinesia
• Sinus infections, bronchiectasis, decreased female fertility and absent male fertility
• Dynein mutation
• Dextrocardia

Question 16

Chediak Higashi

Answer 16

LYST mutatin which is normally requred for MT trafficking and endosomal sorting, leads to
-Immunodeficeny, albinism, and neuropathy