5/19 Flashcards
1
Q
tRNA
A
- Binds to amino acid at 3’ end and has CCA at each 3’ end that binds AA
- Reads complementary to mRNA
2
Q
Amino-acyl tRNA synthestase
A
- Is necessary for the correct AA pairing with the right tRNA
- Uses ATP to form bond between tRNA and AA, this energy is what is used to catalyze peptide bond formation
- Aminoacyl tRNA is charged and can bind A site (inhibited by tetracyclines)
3
Q
Initition
A
Requires intiating factors to bind ribosome and hold structure
-Once mRNA is bound then initation factors fall off
4
Q
Elongation
A
- aminoacyl tRNA binds to A site on 30s, bond is catalyzed in P site and moves off in E site
- GTP is used for the translocation step
5
Q
Termination
A
Poly A tail is necessary for termination,
Stop codons UAA, UGA,UAG
6
Q
Antibiotics
A
- Aminoglycosides bind 30S and cause misreading at initiation
- Macrolides bind 50S and prevent leaving of tRNA
- Chloramphenicol blocks transpeptidation
7
Q
RER
A
- N linked sugar added
- Also site of collagen hydroxylation
- Enriched in mucus cells and plasma cells
8
Q
Golgi
A
-N linked sugars and modification of O linked
-Attachement of M6P and other signals
-Clathrin is from cell surface, COPI is in and COPII is out
-
9
Q
I cell diesease
A
- Golgi can’t add M6P and so can’t send lyosomal enzymes to lysosome
- Increased enzymes in blood, cluding of corneas, joint immobility,
10
Q
Angelmanns Symdrome
A
- inaproporiate laaughter, imprinting of fathers leading to only expression of mothers
- Happy demeanor, siezures, retardation
- Protein is an E3 ligase
11
Q
VHL
A
- Vascular tumors and siezures
- mutation is E3 ligase
12
Q
Microtubule Drugs
A
Vincristine, Blastine, mebednazole, griseofulvin all destabalize
-Paclitaxel stabalizes in locked position
13
Q
Vimentin
A
Connective Tissue
14
Q
Keratin
A
Epithelium
15
Q
Karterengers
A
- Ciliary dyskinesia
- Sinus infections, bronchiectasis, decreased female fertility and absent male fertility
- Dynein mutation
- Dextrocardia
