4d p2

Question 1

How is gel electrophoresis used to find genetic diseases in DNA samples?

Answer 1

DNA screening is used to determie geneitc diseases, then Once DNA sample is obtained, gel electrophoresis can be used if the disease-causing allele is present

Question 2

Purpose of Genetic testing

Answer 2

To confirm a genetic disorder
Determine if a person has a gene which predisposes them toward developing a disease such as breast cancer

Question 3

What is the difference between a normal allele and a mutant allele in terms of MstII restriction enzyme recognition?

Answer 3

Normal allele, restriction enzyme MstII cuts a particular gene forming 2 fragments
fragement 1: 100bp
fragment 2: 300bp

Mutant allele for sickle cell anaemia, restriction enzyme MstII does not cut the gene
fragment:400bp

Question 4

what is the mutation associated with cystic fibrosis(CF)

Answer 4

3 nucleotides are deleted in the CFTR gene

Question 5

what kind of trait is cystic fibrosos

Answer 5

It is a recessive trait which means individuals must have two alleles impacted with CF

Question 6

vWhen is sample DNA collected for analysis in the context of Cystic Fibrosis diagnosis?

Answer 6

Sample DNA is collected after birth which then undergoes PCR and gel electrophoresis

Question 7

how are carriers of an alle can be indeitiifed

Answer 7

gels

Question 8

DNA profiling/dna fingerptign

Answer 8

The process of identification using genetic information.

Also known as DNA fingerprinting.

Question 9

purpose of dna profiling

Answer 9

To identify criminal in forensic investigation
Determine if families are biologically related (custody)
Identifying bodies

Question 10

Short tandem repeats (STRs)

Answer 10

STRs are small sections of repeated nucleotides found in the non-coding regions of autosomal chromosomes

Question 11

What does PCR stand for, and what is its purpose in DNA analysis?

Answer 11

PCR stands for Polymerase Chain Reaction, and its purpose in DNA analysis is to amplify small amounts of DNA to create larger quantities for further analysis like genetic testing

Question 12

In repeating Short tandem repeats, in section 1 and 2 shows AATG repeated how many times

Answer 12

AATG is repeated 5 times in sample 1

AATG is repeated 8 times in sample 2

Question 13

In a parental test, how much of the STR repeats does the child inherit

Answer 13

a child inherits half the short tandem repeats if their STRS are from their parents

Question 14

Social implications of DNA profiling

Answer 14

Benefits of DNA profiling socially: Helps to identify and convict perpreators crimes

Consequences:
Privacy concerns, who will have access to the data?

Question 15

Ethical implications of DNA profiling

Answer 15

benefitting of DNA profiling: Can determine lineage and can determine organ donors and patients

Consequences of DNA profiling:

Personal data may be leaked and resulted are not always relibable because DNA can get contamintaed.