4.7.2013(micro-gram Positive Bacteria) Flashcards
Temperature of culture of staph for pigment production
22 C
Selective medium for staphylococci
Salt milk agar(8-10% Nacl)
Mannitol salt agar(7.5% Nacl)
Ludlam Lithium chloride and tellurite media
Cause for golden yellow pigmentation of s.aureus colonies
Carotene
Gram positive bacteria that produces endotoxins
Listeria
Gram negative cocci
Neisseria
Veilonella
Gram positive bacteria with pili
Corynebacterium renale
Catalase positive gram positive cocci
S.aureus
Micrococci
Diff btw staph and Micrococci
Micrococci are
Oxidase Positive
Furazolidone resistant
Bacitracin sensitive
Staph which is slide coagulase positive but tube coagulase negative
S.lugdunensis
Slide coagulase positivity is because of
Bound coagulase(clumping factor)
Tube coagulase test requires free coagulase which needs coagulase reacting factor in plasma
Which is a virulence factor free or bound coagulase?
Free coagulase(it is heat Labile)
Basis of coagglutination reaction
Protein A of staph.aureus which binds to Fc fragment of IgG
Most consistent association of virulence of S.aureus
Coagulase
Fermentation of mannitol
Factor of S.aureus that exhibits hot cold phenomenon
Beta hemolysin
Coagulase acts by
Activation of prothrombin
Toxin associated with community acquired MRSA
Leukocidin/panton valentine toxin
Hemolysins in S.aureus
A,B,G,D
Vomiting in staph food poisoning
Activation of vagus and vomiting centre
The S.aureus toxin that is inactivated at 70C and activated paradoxically at 100 c
Alpha Hemolysins
At 100 c an inhibitor is inactivated
Bio component toxins of s.aureus
Leukocidin
Gamma hemolysin
Hemolysins of staphylococci that lyses only sheep RBC
Beta hemolysin
Exfoliative toxin of staph belongs to
Bacteriophage II group
Epidermolytic toxin causes
Ritter disease(children) TEN(adults)
Types of Exfoliative toxin of S.aureus
A - heat stable,chromosome mediated
B- heat Labile,plasmid mediated