47 Chapter Flashcards
Model organisms
Species chosen for the ease with which they can be studied in the laboratory.
Fertilization
The formation of a diploid zygote from a haploid egg and sperm.
General events that occur during fertilization
First, sperm dissolve or penetrate any protective layer surrounding the egg to reach the plasma membrane. Next, molecules on the sperm surface bind to receptors on the egg surface, helping ensure that fertilization involves a sperm and egg of the same species. Finally, changes at the surface of the egg prevent polyspermy, a condition in which multiple sperm nuclei enter the egg, fatally disrupting development.
The cell surface events that take place during fertilization have been studied most extensively in sea urchins.
True
Acrosome
A vesicle in the tip of a sperm containing hydrolytic enzymes and other proteins that help the sperm reach the egg.
Acrosomal reaction
The discharge of hydrolytic enzymes from the acrosome when the sperm approaches or contacts an egg.
Fast block to polyspermy
The depolarization of the egg in plasma membrane that begins within 1-3 seconds after a sperm binds to an egg membrane protein. The depolarization lasts about 1 minute and prevents additional sperm from fusing with the egg during that time.
Slow block to polyspermy
The formation of the fertilization envelope and other changes in an egg’s surface that prevent fusion of the egg with more than one sperm. The slow block begins about 1 minute after fertilization.
Cortical reaction
Cortical granules, in the cortex of the egg cytoplasm, lifts the vitelline layer away from the egg and hardens the layer into a protective fertilization envelope. Additional enzymes clip off and release the external portions of the remaining receptor proteins, along with any attached sperm.
Formation of the fertilization envelope requires a high concentration of Ca 2+ in the egg. Why?
First, the binding of the sperm to the egg activates a signal transduction pathway that triggers release of Ca2+ into the cytosol from the endoplasmic reticulum. The resulting increase in Ca2+ levels causes cortical granules to fuse with the plasma membrane.
Fertilization initiates and speeds up metabolic reactions that trigger the onset of embryonic development, “activating” the egg. There is, for example, a marked increase in the rates of cellular respiration and protein synthesis in the egg following fertilization.
True
What marks the end of the fertilization stage?
The first cell division
Zona pellucida
The extracellular matrix surrounding a mammalian egg.
No fast block to polyspermy has been identified in mammals.
True
Overall, the process of fertilization is much slower in mammals than in sea urchins: the first cell division occurs within 12-36 hours after sperm binding in mammals, compared with about 90 minutes in sea urchins. This cell division marks the end of fertilization and the beginning of the next stage of development, cleavage.
True
Cleavage
The succession of rapid cell divisions without significant growth during early embryonic development that converts the zygote to a ball of cells.
Blastomeres
An early embryonic cell arising during the cleavage stage of an early embryo.
Blastula
A hollow ball of cells that marks the end of the cleavage stage during early embryonic development in animals. Consists of the first five to seven cleavage divisions.
Blastocoel
The fluid-filled cavity that forms in the center of a blastula.
Yolk
Nutrients stored in an egg.
In frogs (and many other animals), cleavage is asymmetric, reflecting the asymmetric distribution of yolk across the egg.
True
Vegetal pole
The point at the end of an egg in the hemisphere where most yolk is concentrated; the opposite of animal pole.