4.2.3 Transcription Flashcards
A gene is
a sequence of nucleotide bases in a DNA molecule that codes for the production of a specific sequence of amino acids, that in turn make up a specific polypeptide (protein)
This process of protein synthesis occurs in two stages
- transcription
- translation
During transcription
DNA is transcribed and an mRNA molecule is produced
During translation
mRNA (messenger RNA) is translated and an amino acid sequence is produced
Transcription: this stage of protein synthesis occurs in
the nucleus
Part of a DNA molecule
unwinds (the hydrogen bonds between the complementary base pairs break)
Part of a DNA molecule unwinds, catalysed by
helicase, like in DNA replication
Part of a DNA molecule unwinds, catalysed by helicase, this exposes
the gene to be transcribed (the gene from which a particular polypeptide will be produced)
A complementary copy of the code from the gene is made by
building a single-stranded nucleic acid molecule known as mRNA (messenger RNA)
Free
activated RNA nucleotides
Free activated RNA nucleotides pair up (via hydrogen bonds) with
their complementary (now exposed) bases
Free activated RNA nucleotides pair up (via hydrogen bonds) with their complementary (now exposed) bases on one strand
the template strand
Free activated RNA nucleotides pair up (via hydrogen bonds) with their complementary (now exposed) bases on one strand (the template strand) of
the ‘unzipped’ DNA molecule
The sugar-phosphate groups of these RNA nucleotides are then
bonded together
The sugar-phosphate groups of these RNA nucleotides are then bonded together by the enzyme
RNA polymerase