4. T cell development

Question 1

What is a double negative T cell?

Answer 1

CD3- 4- 8-

Question 2

What is a large active, double positive T cell?

Answer 2

CD3+ pTalpha:beta 4+ 8+

Question 3

What is a small resting, double positive T cell?

Answer 3

CD3+ alpha:beta 4+ 8+

Question 4

What is a small resting single positive T cell?

Answer 4

CD4+8- / CD4- 8+

Question 5

In which order do T cells develop?

Answer 5

double negative
large active double pos
small resting double pos
small resting single pos

Question 6

Which part of the thymus does positive and negative selection occur

Answer 6

medulla

Question 7

What is pre Tau alpha?

Answer 7

A surrogate alpha chain

Question 8

What is CD3 important for?

Answer 8

Signalling

Question 9

Which stage does germline gene configuration occur?

Answer 9

DN1 and 2

Question 10

Which stage does beta chain D-J rearrangement occur?

Answer 10

DN 2 and 4

Question 11

Which stage does beta chain V-DJ rearrangement occur?

Answer 11

DN 3-4

Question 12

At which stage is there a functional beta chain?

Answer 12

DN 4

Question 13

What is the purpose of positive selection?

Answer 13

Ensures all TCR bearing cells can interact with self MHC

Question 14

Where does positive selection take place

Answer 14

Thymis cortical epithelial cells

Question 15

Where does negative selection take place

Answer 15

thymic cortex and medulla

Question 16

CD8 T cell main function

Answer 16

Kill virus -infected cells

Question 17

CD4 Th1 main function

Answer 17

Activate infected macrophages

Provide help to B cells for antibody production

Question 18

CD4 Th2 main function

Answer 18

Drive antibody production, especially switching to IgE

Question 19

CD4 Th17 main function

Answer 19

Enhance neutrophil recruitment

Question 20

CD4 Tfh main function

Answer 20

B cell help - isotope switching

Question 21

CD4 Treg main function

Answer 21

Suppress T cell responses

Question 22

4 Stages of a T cell getting into lymph node

Answer 22

1. Rolling
2. Activation
3. Adhesion
4. Diapedesis

Question 23

What does T cell express during rolling?

Answer 23

Selectins e.g. L selectin

Question 24

What does T cell express during activation?

Answer 24

Chemokines e.g. CCL21

Question 25

What does T cell express during adhesion?

Answer 25

Integrins e.g. LFA 1

Question 26

What regulates diapedesis?

Answer 26

Chemokines

Question 27

Which vascular addressins does L-selectin bind to in high endothelial venules?

Answer 27

CD34

GlyCAM 1

Question 28

Which vascular addressins does L-selectin bind to in mucosal endothelium?

Answer 28

MAdCAM 1

Question 29

Which integrins and adhesion molecules interact in HEV or APC?

Answer 29

LFA-1 and ICAM-1

Question 30

Which integrins and adhesion molecules interact in mucosal endothelium?

Answer 30

LPAM-1 and MAdCAM1

Question 31

Which integrins and adhesion molecules interact in activated endothelium?

Answer 31

VLA-4 and VCAM1

Question 32

3 signals which T cells require to become activated

Answer 32

1. Activation signal - between TCR and MHC and peptide
2. Survival - Co-stimulatory interaction between B7.1/2 and CD28
3. Differentiation signal provided by a secreted cytokine

Question 33

Signal for Treg (2)

Answer 33

TGF beta and -IL6

Question 34

Signal for Tfh

Answer 34

IL6

Question 35

Signal for Th1 (2)

Answer 35

IL12 and IFN gamma

Question 36

Signal for Th2

Answer 36

IL4

Question 37

Signal fo Th17

Answer 37

TGF beta and IL6