4. Blood Typing

Question 1

How did you do this experiment?

Answer 1

• Added 1 drop of anti-A serum (blue) to well A
• 1 drop of anti-B serum (yellow) to well B
• 1 drop of anti-Rh serum (clear) to well Rh
(Closed the cap each time)
• Using dropper vial, placed 1 drop of Sample 1 synthetic blood sample in each well of blood typing slide.
• Used diff color mixing stick for each well (blue for anti-A, yellow for anti-B, white for anti-Rh)

Question 2

WHAT regent did you put in each well?

Answer 2

• Synthetic anti-A serum (blue) to well A
• Synthetic anti-B serum (yellow) to well B
• Synthetic anti-Rh serum (clear) to well Rh

Question 3

HOW do you know if it’s a positive or negative result?

Answer 3

After stirring the synthetic blood + antiserum drops for 30s…
(Positive)
• If there’s clumping/agglomeration → blood reacted w/ specific antibody serum + has antigens that are incompatible with that type of antibody

(Negative)
• No agglutination →blood does NOT have the antigens binding the specific antibody in the reagent