4 Flashcards
how can cancers protect from anti-cancer drugs
upregulate anti-oxidant systems
which amino acids can be quenching systems
glutathiuone, tocopherol, ascorbate
what is the pepetide formula for glutathione
ECG
what is avaulabvlr for redox reactions and conjugation with xenobiottics
cysteine sulfhyrdyrl
where are cojugates transported to in the liver
bile, excreted as mercaptic acid
what are 4 important ions
na ca cl h
what are the two important transpotter families
SLC, solute carrier ABC ATP binding cassette
what do ABCs include
MDR BSEP MRP BCRP
what do SLC include
OATP OAT OCT PEPT
what do SLC include
OATP OAT OCT PEPT
what does SLC6 transport
neurotransmitters
what does SLC22 transportt
organic cations or anions
what is the strcuture of MDRs
N +2x (6 domains +ABC) + C
what shape do MDRs formc
pore that translocates ligands
describe the MDR transport cycle
- open face toward cytosol, ligand binds
- ligand binding causes ABCs to dimerize while pore opens to outside
- ATP hydrolysis
- conformational change reveals inward facing pocket
what is syructure of SLC family proteins
5+5 MOTIF with addtional C or 2 additional N
what are SLC transporters powered by
Na or Cl
what is the transport cycle for SLC
Na and Cl bind in occluded pocket, outward gate open
substrate binds pocket and outward gate closes
transmembrane helices shift removing occlusion, inward gate open releasing Na, Cl, and substrate
outward gate ipens creating open channel, shift
what controls the genes for proteins for angti-oxidant systems
ARE
how does ARE work
Nrf2 translocate to nucleus and promote transciprtion by binding AREs
what is the half life of Nrf2
20 minutes
how does Nrf2 get degraded
ubitiquination
what does Keap 1 dp
localises Cul3 to Nrf2 and sense oxidartive tress
what is Cul3
ubitiquin ligase that tags Nrf2 for degradation
