3.7 Cell Cycle Flashcards

1
Q

Motor proteins use ___ to power transport along the microtubule “railroad”

A

ATP

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2
Q

ATP binding makes actin ___ more easily at the ___ end

A

polymerize
+ end

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3
Q

___ enables cytokines

A

Contractile ring formation of overlapping actin and myosin filaments

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4
Q

ATPase activity increeases once bound in ___

A

Filaments

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5
Q

Myosin moves what?

A

Membranes or cell components

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6
Q

Muscle is contracted by

A

Actin and myosin

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7
Q

Most important cell cycle checkpoints:

A

Enter S phase
Enter Mitosis

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8
Q

Describe “enter S phase” check point

A

Is the environment favorable?
Is it ready to replicate?

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9
Q

Describe the “enter Mitosis” phase

A

Is all DNA replicated?
Is environment favorable?

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10
Q

Phosphorylation allows ___ signals to control ___ protein activity

A

Extracellular
Intracellular

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11
Q

Reversible covalent modifications turn proteins on, off, or both? What can do this?

A

Both

Kinase and phosphatase

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12
Q

Extracellular signals can control intracellular protein activity by activating ___ activators or ___

A

Allosteric activators or inhibitors

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13
Q

The cell cycle is controlled by ___

A

Cdk

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14
Q

Function of Cdk. How does it become active?

A
  • Cdk phosphorylates cell targets that enter different parts of cell cycle
  • Requires cyclin binding to form Cdk complexes (it allosterically binds)
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15
Q

How does Cdk become fully active?

A

Cdk-activating kinase (CAK) phosphorylates Cdk

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16
Q

G1-Cdk is a cyclin type for what role?

A

Advance G1

17
Q

G1/S-Cdk is a cyclin for what phase?

A

Entry into S phase

18
Q

S-Cdk is a cyclin for what phase?

A

DNA replication

19
Q

M-Cdk is a cyclin for what phase?

A

Entry into mitosis

20
Q

What are the 3 ways to control Cdk activity?

A
  • Cyclin concentration
  • Cdk phosphorylation controls activity
  • Cdk inhibitor proteins can inhibit Cdk-cyclin complex formation (cyclin binding)
21
Q

Explain the regulation of cyclin concentration

A
  • Cyclin protein expression
  • Degradation of existing cyclin
22
Q

Explain how Cdk phosphorylation controls activity

A

Activating and inactivating kinases and phosphatases act on Cdk to regulate activity

23
Q

___ control entry into S phase and mitosis

A

Mitogens

24
Q

Steps for mitogen causing release of Myc

A
  1. Mitogen binds to receptor on plasma membrane
  2. Changes conformation and binds to accessory protein (Ras)
  3. Binds to MAP kinase
  4. Causes MAPK cascade
  5. Phosphorylates proteins that can go into nucleus and binds to immediate early genes
  6. Immediate early gene product binds to promoters of early genes
  7. Causes expression of genes (gene regulatory proteins like Myc)
  8. Myc causes expression of cyclins
25
Q

lmmediate early genes expression upregulates transcription of ___

A

Delayed genes

26
Q

The last protein that is phosphorylated by MAP kinase goes where?

A

Into the nucleus

27
Q

What happens if you change the phosphorylation state of a Cdk?

A

It alters Cyclin/Cdk complex activity

28
Q

Cdk is activated by

A

Phosphatase

29
Q

Cdk is inactivated by

A

Kinase

30
Q

___ transcription factor causes expression of genes that drive entry into ___ phase

A

E2F
S phase

31
Q

___ holds E2F inactive until phosphorylated by G1-Cdk

A

Rb (retinoblastoma)

32
Q

Steps for inactivation and activation of E2F

A
  1. Rb inhibits E2F activation
  2. G1 phosphorylates Rb
  3. E2F falls off
  4. E2F is active and starts S phase gene transcription
33
Q

DNA damage halts cell cycle by activating ___

A

p53

34
Q

p53 function

A

detects damaged DNA and stops it G1 from entering S phase in interphase, which prevents replication of bad DNA

35
Q

p53 activates ___ to do what?

A

p21 gene, which encodes an inhibitory protein for G1 and S Cdks

36
Q

What happens if p53 is mutated?

A

There are more damaged cells
- Malignant tumors have inactive p53

37
Q

___ patches chromosome ends

A

Telomerase

38
Q

Define telomerase

A
  • Maintains chromosome length
  • Has its own RNA template and keeps telomeres longer