36-Composititon of saliva + Gingival Crevicular Fluid

Question 1

Composition of saliva

Answer 1

• 99.5% H2O
• 0.5% ⇢ electrolytes, mucous, enzymes + glycoproteins

Question 2

Volume, electrolytes + Peptides

Answer 2

Volume ⇢ 600-1000 ml/day

Electrolytes

• Na⁺ K⁺ Cl^- Ca²⁺
• HCO₃ ^– F^- Mg²⁺

Peptides

• Amylase = Starch ⇢ Glucose + Fructose
• Proline-rich protein
• mucins, histatins, cystatins, peroxidase, lysozyme

Question 3

Immunoglobins, small organic elements, Mucous + other components

Answer 3

Immunoglobins ⇢ IgA, IgG + IgM

Small organic elements

• Glucose AAs Urea Uric acid Enzymes Cholesterol Ammonia

Mucous

• Glycoproteins Serum albumin cAMP-binding protein

Other components

• Epidermal growth factor Insulin

Question 4

Types of saliva

Answer 4

Stimulated saliva

• Response to stimulus at meal time

Unstimulated saliva

• Resting salivary flow

Question 5

Gingival Crevicular Fluid

Answer 5

At gingival margin ⇢ serum flows from inside of gingiva into mouth ⇢ Removes foreign microbes

For resistant populations ⇢ Flow of crevicular fluid provides these organisms w/source of nutrients

• Specialised enzymes break down these proteins to provide them w/nutrients necessary for growth

Question 6

Composition of GCF

Answer 6

Cellular components

• Neutrophils, lymphocytes, monocytes + epithelial cells

Organic ions

• Proteins, albumins + immunoglobins

Inorganic ions

• Na K Ca Mg

Enzymes

• Acid phosphatase alkaline phosphatase dehydrogenase lactoferrin lysozyme

Bacterial products

• Endotoxins acid phosphatase alkaline phosphatase prostaglandin-like products