3.3- MICROSCOPE MEASUREMENTS AND CALCULATIONS

Question 1

When using a light microscope what can we use to measure the size of the objects?

Answer 1

eyepiece graticule

Question 2

What is the eyepiece graticule?

Answer 2

glass disc placed in the eyepiece of a microscope

Question 3

What is etched on the eyepiece graticule?

Answer 3

a scale

Question 4

Typically how long is the scale on the eyepiece graticule?

Answer 4

10mm long

Question 5

In how many sub-division is the scale on the eyepiece graticule divided into?

Answer 5

100 sub-divisions

Question 6

Can the scale on the eyepiece graticule be used directly to measure the size of objects under a microscope’s objective lens?

Answer 6

no

Question 7

Why can’t the scale on the eyepiece graticule be used to directly measure the size of the objects under a microscope’s objective lens?

Answer 7

because each objective lens will magnify to a different degree

Question 8

What must be done for each particular objective lens?

Answer 8

graticule must first be calibrated for particular objective lens

Question 9

What do you need to use when calibrating an eyepiece graticule?

Answer 9

stage micrometre

Question 10

What is etched into the stage micrometre?

Answer 10

a scale

Question 11

Usually how long is the scale on the stage micrometre?

Answer 11

2mm long

Question 12

What is the smallest sub-divisions on the stage micrometre?

Answer 12

0.01mm (10μm)

Question 13

What can you calculate once the eyepiece graticule scale and stage micrometre scales are lined-up?

Answer 13

possible to calculate length of divisions on eyepiece graticule