3.1- METHODS OF STUDYING CELLS Flashcards
What are microscopes?
instruments that produce magnified image of object
What can act as a simple magnifying glass?
simple convex glass lens
How can simple convex glass lenses work more effectively?
when they’re used in pairs in a compound light microscope
What sort of wavelength do light rays have?
long wavelength
What does light rays having long wavelengths mean?
light microscope can only distinguish between two objects if they’re 0.2μm or further apart
How can the limitation of light microscopes only being able to distinguish between two objects if they’re 0.2μm or further apart, be overcome?
by using beams of electrons rather than beams of light
What is the wavelength of electrons like in comparison to wavelength of light rays?
shorter wavelength
What are electron microscopes able to as electrons have a shorter wavelength?
beam of electrons in electron microscope can distinguish between two objects only 0.1nm apart
What is the material that is put under a microscope referred to as?
object
What is the appearance of the material when viewed under the microscope referred to as?
image
What is the magnification of an object?
how many times bigger the image is when compared to the object
magnification equation:
magnification=
size of real objects
What is the resolution or resolving power of a microscope?
minimum distance apart that two objects can be in order for them to appear as separate items
What does the resolving power depend on?
wavelength or form of radiation used
What is the approximate resolution power in a light microscope?
about 0.2 μm
What does a light microscope having a resolving power of 0.2 μm mean?
any two objects which are 0.2 μm or more apart will be seen separately, but any objects closer than 0.2 μm will appear as a single item
What does a greater resolution result in?
greater clarity
image produced is clearer and more precise
How does increasing the magnification affect the size of the image and resolution?
increases the size of the image, but doesn’t always increase the resolution