3.1- METHODS OF STUDYING CELLS Flashcards

1
Q

What are microscopes?

A

instruments that produce magnified image of object

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2
Q

What can act as a simple magnifying glass?

A

simple convex glass lens

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3
Q

How can simple convex glass lenses work more effectively?

A

when they’re used in pairs in a compound light microscope

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4
Q

What sort of wavelength do light rays have?

A

long wavelength

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5
Q

What does light rays having long wavelengths mean?

A

light microscope can only distinguish between two objects if they’re 0.2μm or further apart

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6
Q

How can the limitation of light microscopes only being able to distinguish between two objects if they’re 0.2μm or further apart, be overcome?

A

by using beams of electrons rather than beams of light

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7
Q

What is the wavelength of electrons like in comparison to wavelength of light rays?

A

shorter wavelength

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8
Q

What are electron microscopes able to as electrons have a shorter wavelength?

A

beam of electrons in electron microscope can distinguish between two objects only 0.1nm apart

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9
Q

What is the material that is put under a microscope referred to as?

A

object

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10
Q

What is the appearance of the material when viewed under the microscope referred to as?

A

image

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11
Q

What is the magnification of an object?

A

how many times bigger the image is when compared to the object

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12
Q

magnification equation:

A

magnification=

size of real objects

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13
Q

What is the resolution or resolving power of a microscope?

A

minimum distance apart that two objects can be in order for them to appear as separate items

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14
Q

What does the resolving power depend on?

A

wavelength or form of radiation used

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15
Q

What is the approximate resolution power in a light microscope?

A

about 0.2 μm

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16
Q

What does a light microscope having a resolving power of 0.2 μm mean?

A

any two objects which are 0.2 μm or more apart will be seen separately, but any objects closer than 0.2 μm will appear as a single item

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17
Q

What does a greater resolution result in?

A

greater clarity

image produced is clearer and more precise

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18
Q

How does increasing the magnification affect the size of the image and resolution?

A

increases the size of the image, but doesn’t always increase the resolution

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19
Q

Does every microscope have a limit of resolution?

A

yes

20
Q

What will happen beyond the limit of resolution?

A

increasing the magnification will reveal more detail but beyond this point increasing magnification will not do this
object will appear larger and more blurred

21
Q

What is necessary to study the structure and function of the various organelles?

A

to obtain large numbers of isolated organelles

22
Q

What is cell fractionation?

A

process where cells broken up and different organelles they contain separated out

23
Q

What happens before cell fractionation can begin?

A

tissue placed in cold, buffered solution of same water potential as tissue

24
Q

Why is the solution cold?

A

reduce enzyme activity that might break down the organelles

25
Q

Why does the solution have the same water potential as the tissue?

A

prevent organelles bursting/shrinking due to osmotic gain or loss of water

26
Q

Why is the solution buffered?

A

so the pH doesn’t fluctuate

any change in pH could alter the structure of organelles or affect functioning of enzymes

27
Q

How many stages are there in cell fractionation?

A

two

28
Q

What are the two stages in cell fractionation?

A

homogenation

ultracentrifugation

29
Q

How are cells broken up?

A

by a homogeniser (blender)

30
Q

What does the homogeniser do?

A

release organelles from cell

31
Q

What is the resultant fluid from the homogeniser known as?

A

homogenate

32
Q

What is done with the homogenate?

A

filtered to remove any complete cells and large pieces of debris

33
Q

What is ultracentrifugation?

A

process by which fragments in filtered homogenate separated in machine called centrifuge

34
Q

What does the centrifuge do?

A

spins tubes of homogenate at very high speed in order to create a centrifugal force

35
Q

process of ultracentrifugation of animal cell (1)

A

tube of filtrate placed in centrifuge and spun at low speed

36
Q

process of ultracentrifugation of animal cell (2)

A

heaviest organelles, nuclei, forced to bottom of tube, where they form thin sediment/ pellet

37
Q

process of ultracentrifugation of animal cell (3)

A

fluid at top of tube (supernatant) removed, leaving just sediment of nuclei

38
Q

process of ultracentrifugation of animal cell (4)

A

supernatant transferred to another tube and spun in centrifuge at faster speed than before

39
Q

process of ultracentrifugation of animal cell (5)

A

next heaviest organelles, mitochondria. forced to bottom of tube

40
Q

process of ultracentrifugation of animal cell (6)

A

process continued in this way so that, at each increase in speed, next heaviest organelle sedimented and separated out

41
Q

speed of centrifugation for nuclei?

A

1000 revolutions per min

42
Q

speed of centrifugation for mitochondria?

A

35000 revolutions per min

43
Q

speed of centrifugation for lysosomes?

A

16500 revolutions per min

44
Q

what did the techniques of cell fractionation and ultracentrifugation enable?

A

considerable advances in biological knowledge

45
Q

What did cell fractionation and ultracentrifugation allow?

A

detailed study of structure and function of organelles, by showing what isolated components do