3.1 - importance of ATP + Respiration Flashcards

1
Q

What does ATP consist of?

A

3 inorganic phopshates
pentose sugar (ribose) C5H10O5
nitrogenous base ( adenine)

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2
Q

what is hydrolysis?

A

exergonic

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3
Q

what is condensation?

A

endergonic

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4
Q

what is the synthesis of ATP?

A

phosphorylation

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5
Q

what does ADP stand for ?

A

Adenosine di phosphate

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6
Q

what does AMP stand for?

A

adenosine mono phosphate

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7
Q

what is the equation for ATP?

A

ADP + PI —– ATP

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8
Q

what is ATP synthetase?

A

it is an enzyme that is inside the stalked particle of mitochondrion

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9
Q

what is the equation for respiration?

A

6O2 + C6H1206 —- 6 CO2 + 6H20 + 38 ATP

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10
Q

what are the stages of respiration?

A

Glycosis ( anaerobic)
Link ( aerobic)
Krebs ( aerobic)

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11
Q

what is the equation for photosynthesis?

A

6CO2 + H20 —– H1206+ 602

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12
Q

stages of photosnythesis?

A

light harvesting
light dependent stage
light independent stage

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13
Q

what is chemiosmosis?

A

movement of hydrogen from high to low. It is different concentration in different places

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14
Q

what is he equation?

A

H —- 2H+ + 2e-

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15
Q

Phosphorylation?

A

synthesise ATP
ADP + Pi
reversible reaction
condensation reaction
( reaction when joining)
endergonic ( taking in energy) (30.6KJ mol-1)
movement of H+ (diffusion)
need ATP synthetase

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16
Q

what are glucose + fructose?

A

isomers - same molecular formula but different structure

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17
Q

stages of glycosis?

A

Glucose (6c)
ATP - ADP +Pi
Glucose Phosphate(6c)
ATP - ADP + PI
Fructose biphosphate (6c)

Glycerate - Glycerate -3 - Phosphate
(3c) 3 - Phosphate(3c)
NADH + H2 – NADH2
Pyruvate (3c) Pyruvate (3c)

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18
Q

What is gained and lost ?

A

SLP : 4 ATP
Hydrolysis : -2 ATP
net gain : 2 ATP

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19
Q

Overall from glycolysis?

A

SLP = 2 ATP
OP = 6 ATP
8 FROM GLYCOLYSIS

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20
Q

what is NAD?

A

nicotinoamide
adenine
dinucleotide

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21
Q

Oxidative phosphorlyation?

A

Hydrogen acceptors accepts hydrogen

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22
Q

what is that formula?

A

NAD + H2 — NADH2
NADH2 —– NADH2
H2 —— 2H+ + 2E-
takes place in the matrix into the intermembrane space went through the stalked particle
Phosphorlyated ATP
3 ATP FOR 1 NAD

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25
26
27
what is FAD?
Flavine Adenine Dinucleotide
28
3 types of phosphorylation?
Oxidative Substrate level photophosphorylation?
29
Oxidative?
takes place in the inner membrane of the mitochondria during aerobic respiration energy for making ATP comes from oxidation + reduction (gaining + losing electrons ) along the electron transport chain
30
substrate level?
when phosphate groups are transferred from donor molecules such as glycerate - 3 phosphate to ADP tomake ATP in glycolysis
31
Photophosphorlyation?
occurs on the thylakoid membrane of the chloroplasts in the light dependent stage of photosynthesis energy for making ATP comes from light + is released in the transfer of electrons along a chain of electron carriers Photophosphorlyation does not occur in respiration
32
what is aerobic respiration?
the release if large quantities of energy which can be amde available as ATP from the breakdown of molecules with oxygen as the terminal electron acceptot
33
anaerobic respiration?
the breakdown of molecules in the absence of oxygen releasing less energies so a smaller quantities of ATP is synthesised by SLP
34
obligate aerobe?
most living organisms use aerobic respiration + break down substrates using Oxygen release large quantities of Oxygen
35
Facilitative aerobes?
microorganisms using yeast + bacteria respire aerobically but can respire without Oxygen
36
Obligate aerobes?
species of bacteria + archea use aerobic respiration + cannot grow in the presence of oxygen
37
3 stages of respiration?
Glycolysis link krebs
38
Glycolysis?
occurs in solution in the cytoplasm to generate Pyruvate, ATP + NADH2 reaction occurs in the cytoplasm because glucose cannot pass through the mitochondrial membrane + the enzymes are not present in the mitochondria a glucose molecule = phosphorylated by adding 2 phopshate groups using up 2 ATP making hexose diphosphate Phosphorylated glucose is more reaction so less activation energy for enzyme controlled reactions Phosphorylated glucose is more polar than glucose so less likely to diffuse out of cell Hexosediphosphate is converted to 2 molecules of triose phosphate ( glyceraldelhyde - 3 - phosphate) the 2 triose phosphates are dehydrogenated oxidising them to pyruvate (3c) the hydrogen atoms = transferred to NAD which is a hydrogen carrier each NAD can produce 3 ATP as the hydrogen ions pass through 3 proton pumps the 2 triphosphate molecules = dehydrogenated and oxidised to pyruvate
39
part 2?
hydrogen atoms = transferred to NAD forming reduced (NADH2) these steps release 4 ATP molecules by substrate level phosphorylation 4 ATP = synthesised but 2 were phosphorylated from glucose so there = only a net gain of 2 ATP by SLP 2 molecules of NADH2 = produced only if oxygen is available each molecule of NAD produces 3 ATP same energy is lost as heat Substrate level phosphorylation C6H1206 + 2 NAD + 2 ADP + 2Pi ------ 2 pyruvate + 2NADH NADH2 -------- NAD + H2 H2(g) ---- 2H+2e- Hydrogen ions pass through proton pumps by Active transport into the intermembrane space H+ ions diffuse through the stalked particle producing 30.6 KJ mol-1 of energy which allows ADP and phosphate to form ATP reaction is catalysed by ATP synthetase
40
Link reaction?
pyruvate diffuses from the cytoplasm Pyruvate (3c) NADH2 CO2 Acetate (2c) Pyruvate is dehyrogenated + decarboxylatd to form acetate 2c Acetate combines with co enzyme A to firm acetyl CO -A ATP = phosphORylated by oxidative phosphorylaton using NADH2
41
Krebs?
oxaloacetate (2c) combines with Acetyl CO - A to form citrate citrate is dehydrogenated * NADH2 ----- NADH + H2) ATP synthesised as there are 2 pyruvate molecules citrate is decarboxylated to a 5 carbon compound 5 carbon compound is decarboxylated + dehydrogenated and only 1 ATP is produced by substrate level phosphorylation 4 carbon compound is dehydrogenated using FAD as hydrogen acceptor + generating 2x2 = 4 4 carbon compound is regenerated to reduce Oxygen and undergoes dehydration 2 enzymes involved in decarboxylation are decarboxylase + dehydrogensae 12 ATP = produced per cycle but there are 2 cycles
42
Electron transport chain?
ETC is located in the cristae of the inner mitochondrial membrane it is a series of protein molecules which are either carriers or pumps in intirinsic proteins ; they are sometimes known as respiratory enzymes the carrier molecules include cytochrome, so the ETC can be called the cytochrome chain these reactions release energy which is carried in the form of ATP Hydrogen atoms are carried to the ETC by hydrogen carriers ( co-enzymes) NAD and FAD NAD feeds electrons and protons into the ETC at an earlier stage than FAD NAD has enough energy to synthesise 3 ATP whereas FAD passes H2 directly into the 2nd proton pump Electrons from NADH2 and FADH2 are passed through electron carriers to produce water protons from NAD + FAD are pumped into the inter - membrane space by active transport and flow through the stalked particle which contains ATP synthetase which allows the synthesis of ATP
43
Cytochromes?
proteins containing iron or copper
43
Electrons?
NADH2 donates the electrons from the H2 atoms to the first electron carrier Electrons provide the pumps with energy at the end of the chain, electrons combine with peoton and oxygen to form water 2H+ + e- + 1/2 O2 --- H20
43
Anaerobic respiration - without Oxygen
if there is no O2 to remove H2 from NAD, electron transport chain cannot function there is no oxidative phosphorylation NADH2 cannot be reoxidised so no NAD is regenrated so no more Hydrogen can be picked up Link + Krebs cannot take place the only stage possible is glycolysis but for this to happen pyruvate and hydrogen must be constantly removed + NAD mut be regenerated Pyruvate accepts Hydrogen from NADH2 only SLP can take place
44
Animals?
muscle cells may not get sufficient oxygen during vigorous exercise when deprived of Oxygen, pyruvate becomes a hydrogen acceptor and is converted to lactate lactic acid regenerating NAD if O2 becomes available, the lactate can be respired into CO2 + H2O+ release energy
45
Plant cells + fungi?
under certain conditions, such as water logged soil, pyruvate is converted to CO2 and ethanal which is a hydrogen acceptor Ethanal is reduced to ethanol. NAD is regenerated This process is not reversible. So even if oxygen is available, ethanol cannot be broken down and accumulated becoming toxic.
46
Energy budget? Glycolysis?
SLP? 4-2 = 2 ATP OP = 3 x 2 = 6 ATP 8 ATP altogether
47
Link?
SLP = 0 OLP = 6 = 3x2
48
Krebs?
SLP = 2 OLP = 22 NAD = 18 FAD = 4
49
How many ATP per glucose?
38
50
What is some ATP used for?
used to move pyruvate or reduce NAD + FAD or molecules may leak through the membrane, so on average only 30 - 32 molecules of ATP are produced per glucose molecule
51
How many joules does 1 mole of glucose produce if completely combusted in oxygen?
2880 KJ
52
for energy to make ATP ?
30.6 x 38 = 1162.8
53
Energy efficiency?
1162.8/2880 x 100 40.4%